A Simple Nano-SiO2-Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts

doi:10.1007/s12161-016-0709-x

	A Simple Nano-SiO2-Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts
	Li, J; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Huzhou Res & Dev Ctr Biol Resources, Huzhou 313000, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ecol Restorat & Biodivers Conservat Key Lab Sichu, Chengdu 610041, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Chengdu 610041, Peoples R China.; Qing, LS; Xie, J; Ding, LS; Li, XD; Xie, J (reprint author), Chengdu Med Coll, Chengdu 610083, Peoples R China.; Wang, QL
刊名	FOOD ANALYTICAL METHODS
	2017
卷号	10 期号:5 页码:1500-1506
关键词	Residue Detection 2 Silicon Dioxide Nanoparticle 4-dichlorophenoxyacetic Acid Direct Competitive Elisa Bean Sprout
DOI	10.1007/s12161-016-0709-x
产权排序	1
文献子类	Article
英文摘要	The abusive use of 2,4-dichlorophenoxyacetic acid (2,4-D) was very common in the commercial production of bean sprouts. Herein, a simple and highly sensitive nano-SiO2-based ELISA method for residual 2,4-D detection was developed. The test depends on the direct competitive combination to anti-2,4-D antibody labeled by horseradish peroxidase (HRP) between nano-SiO2 immobilized by ovalbumin-2,4-D (SiO2-OVA-2,4-D) and free 2,4-D in sample. The linear range of this method was from 1 to 350 ng/mL (r = 0.997), and the limit of detection (LOD) was 0.079 ng/mL. The coefficient variations of intra-day and inter-day were all lower than 10% at three concentrations (20, 150, and 250 ng/mL). The mean recovery was 93.2%, and the specificity was satisfactory. The results indicated that the method could be useful for fast determination of residual 2,4-D in bean sprout samples using classic TMP/HRP system and a conventional UV spectrophotometer.
学科主题	Food Science & Technology
项目编号	中国科学院山地生态恢复与生物资源利用重点实验室
语种	英语
资助机构	Financial support was provided by the National Natural Science Foundation of China (No. 21202161), Innovation Training Program for College Students in Sichuan (No. 201513705078), and Research Program of Education Department of Sichuan (No. 16ZA0299). ; National Natural Science Foundation of China [21202161] ; Financial support was provided by the National Natural Science Foundation of China (No. 21202161), Innovation Training Program for College Students in Sichuan (No. 201513705078), and Research Program of Education Department of Sichuan (No. 16ZA0299). ; National Natural Science Foundation of China [21202161] ; Innovation Training Program for College Students in Sichuan [201513705078] ; Innovation Training Program for College Students in Sichuan [201513705078] ; Research Program of Education Department of Sichuan [16ZA0299] ; Research Program of Education Department of Sichuan [16ZA0299] ; Financial support was provided by the National Natural Science Foundation of China (No. 21202161), Innovation Training Program for College Students in Sichuan (No. 201513705078), and Research Program of Education Department of Sichuan (No. 16ZA0299). ; National Natural Science Foundation of China [21202161] ; Financial support was provided by the National Natural Science Foundation of China (No. 21202161), Innovation Training Program for College Students in Sichuan (No. 201513705078), and Research Program of Education Department of Sichuan (No. 16ZA0299). ; National Natural Science Foundation of China [21202161] ; Innovation Training Program for College Students in Sichuan [201513705078] ; Innovation Training Program for College Students in Sichuan [201513705078] ; Research Program of Education Department of Sichuan [16ZA0299] ; Research Program of Education Department of Sichuan [16ZA0299]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29242]
专题	成都生物研究所_天然产物研究
通讯作者	Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Huzhou Res & Dev Ctr Biol Resources, Huzhou 313000, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Ecol Restorat & Biodivers Conservat Key Lab Sichu, Chengdu 610041, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Chengdu 610041, Peoples R China.; Xie, J (reprint author), Chengdu Med Coll, Chengdu 610083, Peoples R China.
推荐引用方式 GB/T 7714	Li, J,Qing, LS ,Qing, LS ,et al. A Simple Nano-SiO2-Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts[J]. FOOD ANALYTICAL METHODS,2017,10(5):1500-1506.
APA	Li, J.,Qing, LS .,Qing, LS .,Qing, LS .,Qing, LS.,...&Wang, QL.(2017).A Simple Nano-SiO2-Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts.FOOD ANALYTICAL METHODS,10(5),1500-1506.
MLA	Li, J,et al."A Simple Nano-SiO2-Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts".FOOD ANALYTICAL METHODS 10.5(2017):1500-1506.