Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine

doi:10.1016/j.talanta.2017.03.011

	Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine
	Xie, J; Li, XD; Ding, LS; Liang, J; Luo, P; Qing, LS; Luo, P (reprint author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Nat Med & Translat Med, Chengdu 610041, Peoples R China.; Wang, QL
刊名	TALANTA
	2017
卷号	167 期号:2017 页码:617-622
关键词	Enzyme-linked Ligand Binding Assay Nano-sio2 Ibuprofen Direct Competitive Hsa
DOI	10.1016/j.talanta.2017.03.011
产权排序	1
文献子类	Article
英文摘要	The application domains of classic enzyme-linked ligand binding assay (ELBA) is relatively narrow due to the high cost and hardly available binding receptor. In here, we described for the first time the possibility of developing a new ELBA based on silica nanoparticles (nano-SiO2) to assess the ibuprofen in human urine. Nano-SiO2 with a large surface area was introduced as stationary phase to improve the analytical performance. In the experiment, a competitively binding procedure with human serum albumin (HSA) was performed between the ibuprofen presented in sample and horseradish peroxidase labeled ibuprofen (HRP-ibuprofen) subsequently added. After centrifugal separation, the HRP/ibuprofen/nano-SiO2 composite catalyzed the substrate solution (TMB/H2O2) with a color change from colorless to yellow for quantitative measurement via an ultraviolet spectrophotometer. As a validation of the new principle, the developed nano-ELBA method was applied in the determination of ibuprofen excreted in human urine with excellent performance. This detection range only depends on the solubility of ligand and sensitivity of UV spectrophotometer. Our results indicate that this new method demonstrated to be able to rapidly and adequately determine the concentration of components in biological samples and advocate its effectiveness for various applications.
学科主题	Chemistry
项目编号	中国科学院山地生态恢复与生物资源利用重点实验室
语种	英语
资助机构	This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Macao Science and Technology Development Fund [052/2013/A2] ; Macao Science and Technology Development Fund [052/2013/A2] ; This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; This work was supported by the National Natural Science Foundation of China (No. 21202161) and the Macao Science and Technology Development Fund (No. 052/2013/A2). ; National Natural Science Foundation of China [21202161] ; Macao Science and Technology Development Fund [052/2013/A2] ; Macao Science and Technology Development Fund [052/2013/A2]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29237]
专题	成都生物研究所_天然产物研究
通讯作者	Luo, P (reprint author), Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China.; Qing, LS (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Nat Med & Translat Med, Chengdu 610041, Peoples R China.
推荐引用方式 GB/T 7714	Xie, J,Li, XD,Ding, LS,et al. Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine[J]. TALANTA,2017,167(2017):617-622.
APA	Xie, J.,Li, XD.,Ding, LS.,Liang, J.,Luo, P.,...&Wang, QL.(2017).Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine.TALANTA,167(2017),617-622.
MLA	Xie, J,et al."Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine".TALANTA 167.2017(2017):617-622.