Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis

doi:10.1038/srep23330

	Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis
	Kang, L (reprint author), Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.; Chen, B; Zhang, Y; Zhang, X; Jia, SL; Chen, S; Kang, L; Kang, L (reprint author), Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China.
刊名	SCIENTIFIC REPORTS
	2016
卷号	6 页码:23330（1-8）
DOI	10.1038/srep23330
产权排序	3
文献子类	Article
英文摘要	An increasing number of long noncoding RNAs (lncRNAs) have been discovered with the recent advances in RNA-sequencing technologies. lncRNAs play key roles across diverse biological processes, and are involved in developmental regulation. However, knowledge about how the genome-wide expression of lncRNAs is developmentally regulated is still limited. We here performed a whole-genome identification of lncRNAs followed by a global expression profiling of these lncRNAs during development in Drosophila melanogaster. We combined bioinformatic prediction of lncRNAs with stringent filtering of protein-coding transcripts and experimental validation to define a high-confidence set of Drosophila lncRNAs. We identified 1,077 lncRNAs in the given transcriptomes that contain 43,967 transcripts; among these, 646 lncRNAs are novel. In vivo expression profiling of these lncRNAs in 27 developmental processes revealed that the expression of lncRNAs is highly temporally restricted relative to that of protein-coding genes. Remarkably, 21% and 42% lncRNAs were significantly upregulated at late embryonic and larval stage, the critical time for developmental transition. The results highlight the developmental specificity of lncRNA expression, and reflect the regulatory significance of a large subclass of lncRNAs for the onset of metamorphosis. The systematic annotation and expression analysis of lncRNAs during Drosophila development form the foundation for future functional exploration.
学科主题	Science & Technology - Other Topics
语种	英语
资助机构	National Science Foundation of China [31472048, 31430023, 11171088] ; National Science Foundation of China [31472048, 31430023, 11171088] ; Province Natural Science Foundation of Hebei [A2015208108] ; Province Natural Science Foundation of Hebei [A2015208108] ; National Science Foundation of China [31472048, 31430023, 11171088] ; National Science Foundation of China [31472048, 31430023, 11171088] ; Province Natural Science Foundation of Hebei [A2015208108] ; Province Natural Science Foundation of Hebei [A2015208108]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/28203]
专题	成都生物研究所_天然产物研究
通讯作者	Kang, L (reprint author), Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China.; Kang, L (reprint author), Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China.
推荐引用方式 GB/T 7714	Kang, L ,Chen, B,Zhang, Y,et al. Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis[J]. SCIENTIFIC REPORTS,2016,6:23330（1-8）.
APA	Kang, L .,Chen, B.,Zhang, Y.,Zhang, X.,Jia, SL.,...&Kang, L .(2016).Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis.SCIENTIFIC REPORTS,6,23330（1-8）.
MLA	Kang, L ,et al."Genome-wide identification and developmental expression profiling of long noncoding RNAs during Drosophila metamorphosis".SCIENTIFIC REPORTS 6(2016):23330（1-8）.