Sequence-Specific Biosensing of DNA Target through Relay PCR with Small-Molecule Fluorophore

doi:10.1021/acschembio.5b01081

	Sequence-Specific Biosensing of DNA Target through Relay PCR with Small-Molecule Fluorophore
	Tang, Z; Yasmeen, A; Du, F; Zhao, YY; Dong, J; Chen, HD; Huang, X; Cui, X; Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China.
刊名	ACS CHEMICAL BIOLOGY
	2016
卷号	11 期号:7 页码:1945-1951
DOI	10.1021/acschembio.5b01081
产权排序	1
文献子类	Article
英文摘要	Polymerase chain reaction coupled with signal generation offers sensitive recognition of target DNA sequence; however, these procedures require fluorophore-labeled oligonucleotide probes and high-tech equipment to achieve high specificity. Therefore, intensive research has been conducted to develop reliable, convenient, and economical DNA detection methods. The relay PCR described here is the first sequence-specific detection method using a small molecule fluorophore as a sensor and combines the classic 5'-3' exonuclease activity of Taq polymerase with an RNA mimic of GFP to build a label-free DNA detection platform. Primarily, Taq polymerase cleaves the 5' noncomplementary overhang of the target specific probe during extension of the leading primer to release a relay oligo to initiate tandem PCR of the reporting template, which encodes the sequence of RNA aptamer. Afterward, the PCR product is transcribed to mRNA, which could generate a fluorescent signal in the presence of corresponding fluorophore. In addition to high sensitivity and specificity, the flexibility of choosing different fluorescent reporting signals makes this method versatile in either single or multiple target detection.
学科主题	Biochemistry & Molecular Biology
语种	英语
资助机构	National Natural Science Foundation of China [21172215, 21102140, 21402189, 21322208] ; National Natural Science Foundation of China [21172215, 21102140, 21402189, 21322208] ; National Natural Science Foundation of China [21172215, 21102140, 21402189, 21322208] ; National Natural Science Foundation of China [21172215, 21102140, 21402189, 21322208]
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/28189]
专题	成都生物研究所_天然产物研究
通讯作者	Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China.
推荐引用方式 GB/T 7714	Tang, Z,Yasmeen, A,Du, F,et al. Sequence-Specific Biosensing of DNA Target through Relay PCR with Small-Molecule Fluorophore[J]. ACS CHEMICAL BIOLOGY,2016,11(7):1945-1951.
APA	Tang, Z.,Yasmeen, A.,Du, F.,Zhao, YY.,Dong, J.,...&Tang, Z .(2016).Sequence-Specific Biosensing of DNA Target through Relay PCR with Small-Molecule Fluorophore.ACS CHEMICAL BIOLOGY,11(7),1945-1951.
MLA	Tang, Z,et al."Sequence-Specific Biosensing of DNA Target through Relay PCR with Small-Molecule Fluorophore".ACS CHEMICAL BIOLOGY 11.7(2016):1945-1951.