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	RhPDGF-BB Inhibits Bone Loss via Balancing Osteogenesis and Adipogenesis in Steroid Associated Osteoporotic Rabbits
	Huijuan Cao; Jing Long; Keda Shi; Tianluo Ye; Cuishan Huang; Ling Li; Yuxiao Lai; Ling Qin; Xinluan Wang
	2017
会议日期	2017
会议地点	广东珠海
英文摘要	Objective: Recombinant human platelet-derived growth factor (rhPDGF-BB) is a clinically proven growth factor with the potential of promoting cell proliferation and osteogenic differentiation during the bone regeneration process. This study was to prove our hypothesis that rhPDGF-BB was able to prevent bone mass loss in a steroid associated osteoporosis (SAOP) rabbit model, meanwhile we employed the mouse embryonic mesenchymal stem cells C3H10T1/2 to investigate the mechanism of rhPDGF-BB for treatment of SAOP. Methods: SAOP was induced in rabbits by injection of liposaccharide (LPS) and methylprednisolone (MPS) according to our established protocol. At 2, 4, 6 weeks after induction, 2g rhPDGF-B，25g Anti-PDGF-BB and saline were intramedullary injected into the bone marrow of proximal femur respectively. At week 6 after induction, distal femora were dissected for micro-CT testing. Meanwhile, the C3H10T1/2 cells were cultured with osteogenic or adipogenic differentiation medium. Meantime, the experimental cells were treated with 50 ng/ml rhPDGF-BB, 312.5 ng/ml Anti-PDGF-BB or saline. During differentiation progression, we analyzed the osteoblastic differentiation markers and mineralization, as well as the adipogenic differentiation markers and oil droplets formation and accumulation in C3H10T1/2 cells. Results: Micro-CT results showed that rhPDGF-BB could enhance the BMD, BV/TV, and Tb.Th of cancellous bone of distal femur, as well as improve the cross-sectional thickness of cortical bone in SAOP rabbit model. Meanwhile, rhPDGF-BB significantly enhanced C3H10T1/2 cells proliferation, osteogenic differentiation and calcium deposition in vitro. In contrast, the adipogenesis of C3H10T1/2 cells was suppressed in the presence of rhPDGF-BB. Conclusion: rhPDGF-BB inhibits bone loss via balancing osteogenesis and adipogenesis in SAOP rabbits. Acknowledge: This work was supported by Ministry of Science and Technology (Project No.2015DFG32200), Natural Science Foundation of China (Project No.81501893), Shenzhen Sino-Swiss Science and Technology Cooperation Project (Project No.GJHZ20150316143827260) and Shenzhen Fundamental Research Foundation (Project No. JCYJ20160229195715386).
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/12249]
专题	深圳先进技术研究院_医工所
作者单位	2017
推荐引用方式 GB/T 7714	Huijuan Cao,Jing Long,Keda Shi,et al. RhPDGF-BB Inhibits Bone Loss via Balancing Osteogenesis and Adipogenesis in Steroid Associated Osteoporotic Rabbits[C]. 见:. 广东珠海. 2017.

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