Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells

doi:10.1107/S2052252517017912

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells
	Yao, SK ; Fan, JD ; Chen, ZY ; Zong, YB ; Zhang, JH ; Sun, Z ; Zhang, L ; Tai, R ; Liu, Z ; Chen, C
刊名	IUCRJ
	2018
卷号	5 页码:141-149
关键词	X-ray Tomography Fluorescence Microscopy Tumor Microenvironment Fullerene Derivatives Electron Tomography Nanoparticles Absorption 3d Macrophage Resolution
ISSN号	2052-2525
DOI	10.1107/S2052252517017912
文献子类	期刊论文
英文摘要	Knowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography ( EST). Macrophages with a size of similar to 20 mu m that had been exposed to the potential antitumour agent [Gd@C-82(OH)(22)](n) were investigated. Large numbers of nanoparticles (NPs) aggregated within the cell and were mainly located in phagosomes. No NPs were observed in the nucleus. Imaging of the nanomedicine within whole cells advanced the understanding of the highefficiency antitumour activity and the low toxicity of this agent. This imaging technique can be used to probe nanomaterials within intact large-size cells at nanometre resolution uniformly in three dimensions and may greatly benefit the fields of nanomedicine and nanotoxicology.
语种	英语
WOS记录号	WOS:000427041400006
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/29191]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Yao, SK 2.Fan, JD 3.Chen, ZY 4.Zong, YB 5.Zhang, JH 6.Sun, Z 7.Zhang, L 8.Tai, R 9.Liu, Z 10.Chen, C
推荐引用方式 GB/T 7714	Yao, SK,Fan, JD,Chen, ZY,et al. Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells[J]. IUCRJ,2018,5:141-149.
APA	Yao, SK.,Fan, JD.,Chen, ZY.,Zong, YB.,Zhang, JH.,...&Jiang, H.(2018).Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.IUCRJ,5,141-149.
MLA	Yao, SK,et al."Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells".IUCRJ 5(2018):141-149.