CsPDC-E1 alpha, a novel pyruvate dehydrogenase complex E1 alpha subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth

doi:10.1139/cjps-2017-0074

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	CsPDC-E1 alpha, a novel pyruvate dehydrogenase complex E1 alpha subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth
	Pan, JT ; Li, DQ ; Shu, ZF ; Jiang, X ; Ma, WW ; Wang, WD ; Zhu, JJ ; Wang, YH
刊名	CANADIAN JOURNAL OF PLANT SCIENCE
	2018
卷号	98 期号:1 页码:62-70
关键词	Developmental Expression Photosynthetic Activity Stress Germination Mitochondria Metabolism Tolerance Toxicity Acid
ISSN号	0008-4220
DOI	10.1139/cjps-2017-0074
文献子类	期刊论文
英文摘要	Cadmium (Cd) is one of the most toxic heavy metal pollutants highly hazardous to pollen tubes by disrupting mitochondria. Mitochondrial pyruvate dehydrogenase complex (PDC) plays an important role in cellular metabolism and links cytosolic glycolytic metabolism to the tricarboxylic acid cycle. However, the relationship between PDC, pollen germination, and pollen tube growth under Cd stress remains unclear. Here, we found that Cd inhibited Camellia sinensis (L.) Kuntze pollen germination and pollen tube growth in a dose-dependent manner and disrupted the tip clear zone of the pollen tube. Furthermore, we isolated a novel PDC gene (CsPDC-E1 alpha) from C. sinensis. The full-length cDNA of CsPDC-E1 alpha was 1606 bp and encoded a 393 amino acid protein containing a typical PDC thiamine pyrophosphate (TPP) binding site, heterodimer interface, phos-phorylation loop region, and tetramer interface domain, suggesting that CsPDC-E1 alpha was a member of the PDC_ADC_BCADC subfamily in the TPP family. Bioinformatics analysis indicated that the CsPDC-E1 alpha protein shared a high degree of homology with that from Petunia x hybrida. The CsPDC-E1 alpha relative expression levels in pollen were significantly higher than other tissues of C. sinensis, indicating that CsPDC-E1 alpha expression is tissue-specific. To confirm the functions of CsPDC-E1 alpha in pollen response to Cd stress, we analyzed the relative expression level of CsPDC-E1 alpha in Cd-treated pollen tubes and found that the expression of CsPDC-E1 alpha was induced by Cd stress. All these results indicate that CsPDC-E1 alpha might be associated with Cd inhibition of C. sinensis pollen germination and pollen tube growth.
语种	英语
WOS记录号	WOS:000424449800008
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/28952]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Pan, JT 2.Li, DQ 3.Shu, ZF 4.Jiang, X 5.Ma, WW 6.Wang, WD 7.Zhu, JJ 8.Wang, YH
推荐引用方式 GB/T 7714	Pan, JT,Li, DQ,Shu, ZF,et al. CsPDC-E1 alpha, a novel pyruvate dehydrogenase complex E1 alpha subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth[J]. CANADIAN JOURNAL OF PLANT SCIENCE,2018,98(1):62-70.
APA	Pan, JT.,Li, DQ.,Shu, ZF.,Jiang, X.,Ma, WW.,...&Wang, YH.(2018).CsPDC-E1 alpha, a novel pyruvate dehydrogenase complex E1 alpha subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth.CANADIAN JOURNAL OF PLANT SCIENCE,98(1),62-70.
MLA	Pan, JT,et al."CsPDC-E1 alpha, a novel pyruvate dehydrogenase complex E1 alpha subunit gene from Camellia sinensis, is induced during cadmium inhibiting pollen tube growth".CANADIAN JOURNAL OF PLANT SCIENCE 98.1(2018):62-70.