PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe

doi:10.1021/acsami.7b11994

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe
	Yang, X; Wen, YL; Wang, LL; Zhou, CQ; Li, Q; Xu, L; Li, LY; Shi, JY; Lal, R; Ren, SZ
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017
卷号	9 期号:44 页码:38281-38287
关键词	Sequence-specific Detection Highly Sensitive Detection Ovarian-cancer Incidence Gold Nanoparticles Electrochemical Detection Signal Amplification Biosensing Interface Protein-detection Magnetic Beads Chain-reaction
ISSN号	1944-8244
DOI	10.1021/acsami.7b11994
文献子类	期刊论文
英文摘要	A "sandwich-like" biosensor was developed on the basis of the magnetic bead platform for sensitive detection of breast cancer 1 (BRCA1) DNA. In the present study, a tetrahedron-structured reporter probe (TSRP) was designed, in which 3 vertices of the tetrahedron were labeled with digoxin (Dig), and the other one was labeled with a detection probe. TSRP here provided accurate enzyme loading and well-organized spatial arrangement for optimized signal amplification. The detection limit of this biosensor was as low as 10 fM, which is at least 4 orders of magnitude lower than that of the single DNA probe (100 pM), and the signal gain was 2 times higher than the analysis using three one-dimensional (1D) reporter probes. We could distinguish DNA sequences with only 1 base mismatch, and the performance of our TSRP biosensor was proven to be equally good in both PCR products and real fetal calf serum (FCS) sample as in buffer. We believe this work provided a novel avenue for the development of signal amplification strategies.
WOS关键词	SEQUENCE-SPECIFIC DETECTION ; HIGHLY SENSITIVE DETECTION ; OVARIAN-CANCER INCIDENCE ; GOLD NANOPARTICLES ; ELECTROCHEMICAL DETECTION ; SIGNAL AMPLIFICATION ; BIOSENSING INTERFACE ; PROTEIN-DETECTION ; MAGNETIC BEADS ; CHAIN-REACTION
语种	英语
WOS记录号	WOS:000415140800015
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/28767]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Yang, X,Wen, YL,Wang, LL,et al. PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(44):38281-38287.
APA	Yang, X.,Wen, YL.,Wang, LL.,Zhou, CQ.,Li, Q.,...&Liu, G.(2017).PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe.ACS APPLIED MATERIALS & INTERFACES,9(44),38281-38287.
MLA	Yang, X,et al."PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe".ACS APPLIED MATERIALS & INTERFACES 9.44(2017):38281-38287.