Ratiometric Fluorescent Probe for Imaging of Pantetheinase in Living Cells

	Ratiometric Fluorescent Probe for Imaging of Pantetheinase in Living Cells
	Hu, Yiming 1,2; Li, Hongyu 1; Shi, Wen 1; Ma, Huimin 1,2
刊名	ANALYTICAL CHEMISTRY
	2017-10-17
卷号	89 期号:20 页码:11107-11112
英文摘要	Pantetheinase, which catalyzes the cleavage of pantetheine to pantothenic acid (vitamin B5) and cysteamine, is involved in the regulation of oxidative stress, pantothenate recycling and cell migration. However, further elucidating the cellular function of this enzyme is largely limited by the lack of a suitable fluorescence imaging probe. By conjugating pantothenic acid with cresyl violet, herein we develop a new fluorescence probe CV-PA for the assay of pantetheinase. The probe not only possesses long analytical wavelengths but also displays linear ratiometric (I-628/582 (nm)) fluorescence response to pantetheinase in the range of 5-400 ng/mL With a detection limit of 4.7 ng/mL. This probe has been used to evaluate the efficiency of different inhibitors and quantitatively detect pantetheinase in serum samples, revealing that pantetheinase in fetal bovine serum and new born calf serum is much higher than that in normal human serum. Notably, with the probe the ratiometric imaging and in situ quantitative comparison of pantetheinase in different living cells (LO2 and HK-2) have been achieved for the first time. It is found that the level of pantetheinase in LO2 cells is much larger than that in HK-2 cells, as further validated by Western blot analysis. The proposed probe may be useful to better understand the specific function of pantetheinase in the pantetheinase-related pathophysiological processes.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/41313]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yiming,Li, Hongyu,Shi, Wen,et al. Ratiometric Fluorescent Probe for Imaging of Pantetheinase in Living Cells[J]. ANALYTICAL CHEMISTRY,2017,89(20):11107-11112.
APA	Hu, Yiming,Li, Hongyu,Shi, Wen,&Ma, Huimin.(2017).Ratiometric Fluorescent Probe for Imaging of Pantetheinase in Living Cells.ANALYTICAL CHEMISTRY,89(20),11107-11112.
MLA	Hu, Yiming,et al."Ratiometric Fluorescent Probe for Imaging of Pantetheinase in Living Cells".ANALYTICAL CHEMISTRY 89.20(2017):11107-11112.