Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by beta(2)-adrenergic receptor

CORC > 化学研究所 > 中国科学院化学研究所 > 分子纳米结构与纳米技术实验室

	Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by beta(2)-adrenergic receptor
	Zhang, Mingliang 1,2,3; He, Kangmin 1,2,3; Wu, Jimin 1,2; Li, Nan 3; Yuan, Jinghe 3; Zhou, Wei 3; Ye, Zi 3; Li, Zijian 1,2; Xiao, Han 1,2; Lv, Zhizhen 1,2
刊名	SCIENCE CHINA-CHEMISTRY
	2017-10-01
卷号	60 期号:10 页码:1310-1317
关键词	Transactivation Epidermal Growth Factor Receptor (Egfr) Beta(2)-adrenergic Receptor (Beta(2)-ar) Single Molecule Imaging
英文摘要	Stimulation of G protein-coupled receptors (GPCRs) can lead to the transactivation of the epidermal growth factor receptors (EGFR). The cross-communication between the two signaling pathways regulates several important physiological or pathological processes. However, the molecule mechanism underlying EGFR transactivation remains poorly understood. Here, we aim to study the GPCR-mediated EGFR transactivation process using the single-molecule fluorescence imaging and tracking approach. We found that although EGFR existed as monomers at the plasma membrane of resting cells, they became dimers and thus diffused slower following the activation of beta(2)-adrenergic receptor (beta(2)-AR) by isoproterenol (ISO). We further proved that beta(2)-AR-mediated changes of EGFR in stoichiometry and dynamics were mediated by Src kinase. Thus, the observations obtained via the single-molecule imaging and tracking methods shed new insights into the molecular mechanism of EGFR transactivation at single molecule level.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/39171]
专题	化学研究所_分子纳米结构与纳米技术实验室
作者单位	1.Peking Univ, Inst Vasc Med, Minist Hlth Key Lab Cardiovasc Mol Biol & Regulat, Hosp 3, Beijing 100191, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing Key Lab Cardiovasc Receptors, Beijing 100191, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Mingliang,He, Kangmin,Wu, Jimin,et al. Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by beta(2)-adrenergic receptor[J]. SCIENCE CHINA-CHEMISTRY,2017,60(10):1310-1317.
APA	Zhang, Mingliang.,He, Kangmin.,Wu, Jimin.,Li, Nan.,Yuan, Jinghe.,...&Fang, Xiaohong.(2017).Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by beta(2)-adrenergic receptor.SCIENCE CHINA-CHEMISTRY,60(10),1310-1317.
MLA	Zhang, Mingliang,et al."Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by beta(2)-adrenergic receptor".SCIENCE CHINA-CHEMISTRY 60.10(2017):1310-1317.