Role of Organic Solvents in Immobilizing Fungus Laccase on Single Walled Carbon Nanotubes for Improved Current Response in Direct Bioelectrocatalysis

	Role of Organic Solvents in Immobilizing Fungus Laccase on Single Walled Carbon Nanotubes for Improved Current Response in Direct Bioelectrocatalysis
	Wu, Fei 1,2; Su, Lei.1; Yu, Ping 1,2; Mao, Lanqun 1,2
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2017-02-01
卷号	139 期号:4 页码:1565-1574
英文摘要	Improving bioelectrocatalytic current response of redox enzymes on electrodes has been a focus in the development of enzymatic biosensors and biofuel cells. Herein a mediatorless electroreduction of oxygen is effectively improved in terms of a remarkable enhancement by ca. 600% in maximum reductive current by simply adding 20% ethanol into laccase solution during its immobilization onto single-walled carbon nanotubes (SWCNTs). Conformation analysis by circular dichroism and attenuated total reflectance infrared spectroscopy demonstrate promoted laccase-SWCNTs contact by ethanol, thus leading to favorable enzyme orientation on SWCNTs. Extended investigation on acetone-, acetonitrile-, N,N-dimethylformamide (DMF)-, or dimethyl sulfoxide (DMSO)-treated laccase-SWCNTs electrodes shows a 400% and 350% current enhancement at maxima upon acetone and acetonitrile treatment, respectively, and a complete diminish of reductive current by DMF and DMSO. These results together reveal the important role of organic solvents in regulating laccase immobilization for direct bioelectrocatalysis by balancing surface wetting and protein denaturing.
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/38075]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Fei,Su, Lei.,Yu, Ping,et al. Role of Organic Solvents in Immobilizing Fungus Laccase on Single Walled Carbon Nanotubes for Improved Current Response in Direct Bioelectrocatalysis[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2017,139(4):1565-1574.
APA	Wu, Fei,Su, Lei.,Yu, Ping,&Mao, Lanqun.(2017).Role of Organic Solvents in Immobilizing Fungus Laccase on Single Walled Carbon Nanotubes for Improved Current Response in Direct Bioelectrocatalysis.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,139(4),1565-1574.
MLA	Wu, Fei,et al."Role of Organic Solvents in Immobilizing Fungus Laccase on Single Walled Carbon Nanotubes for Improved Current Response in Direct Bioelectrocatalysis".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139.4(2017):1565-1574.