Functional roles of eriocalyxin B in zebrafish revealed by transcriptome analysis

doi:10.1039/c7mo00125h

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Functional roles of eriocalyxin B in zebrafish revealed by transcriptome analysis
	Zhou, Xunian 1,2; Cao, Cyanne Ye 1; Wan, Angel Tsz-Yau 1; Yue, Grace Gar-Lee 2,3; Kwok, Frankie Hin-Fai 2,3; Fung, Kwok-Pui 1,2,3; Sun, Handong4 ; Lau, Clara Bik-San 2,3; Puno, Pema-Tenzin4 ; Tsui, Stephen Kwok-Wing 1
刊名	MOLECULAR OMICS
	2018-06-01
卷号	14 期号:3 页码:156-169
ISSN号	2515-4184
DOI	10.1039/c7mo00125h
英文摘要	Background. Eriocalyxin B (EriB) is a natural ent-kaurane diterpenoid obtained from Isodon eriocalyx var. laxiflora (family Lamiaceae), which has multiple biological activities (e.g. anti-tumor and anti-inflammatory) via the alteration of gene expression and signaling transduction. Recently, RNA sequencing (RNA-seq) has been developed as a dynamic transcriptome approach to analyze the transcriptional profile and in addition use such gene expression profiles to identify novel candidate genes in a zebrafish model. In the present study, a transcriptome analysis was performed to identify differentially expressed genes (DEGs) in an EriB-exposed zebrafish model. Results. RNA sequencing was conducted on zebrafish embryos after EriB (10 M and 15 M) treatment for 72 h. A total of 1570 (405 up-regulated and 1165 down-regulated) and 2511 genes (543 up-regulated and 1968 down-regulated) were identified in the 10 M and 15 M groups, respectively. Gene ontology analysis was then performed to elucidate the mechanism of action and effects of EriB. We found that 4 pathways were significantly enriched, which include glutathione metabolism, the metabolism of xenobiotics by cytochrome P450, tight junctions, and phototransduction. The critical transcriptional regulators for the DEGs were also identified by Ingenuity Pathway Analysis after the construction of a protein-protein network, which involves p53, c-myc, binding transcription factor 2, sterol regulatory element binding transcription factor 2, nuclear factor erythroid 2 like 2, and interferon regulatory factor 3. Conclusion. In summary, this is the first study to comprehensively explore the effects of EriB in a zebrafish model using a transcriptome analysis approach. Several important genes with substantial changes in expression levels were discovered. The results of this study will provide insights for the future investigation on the biological activities or toxic effects of EriB.
语种	英语
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/61374]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Xunian,Cao, Cyanne Ye,Wan, Angel Tsz-Yau,et al. Functional roles of eriocalyxin B in zebrafish revealed by transcriptome analysis[J]. MOLECULAR OMICS,2018,14(3):156-169.
APA	Zhou, Xunian.,Cao, Cyanne Ye.,Wan, Angel Tsz-Yau.,Yue, Grace Gar-Lee.,Kwok, Frankie Hin-Fai.,...&Tsui, Stephen Kwok-Wing.(2018).Functional roles of eriocalyxin B in zebrafish revealed by transcriptome analysis.MOLECULAR OMICS,14(3),156-169.
MLA	Zhou, Xunian,et al."Functional roles of eriocalyxin B in zebrafish revealed by transcriptome analysis".MOLECULAR OMICS 14.3(2018):156-169.