DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)

doi:10.1111/j.1759-6831.2011.00152.x

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)
	Ren, He 1; Lu, Lu 1,2; Wang, Hong1,2 ; Li, De-Zhu1,2
刊名	JOURNAL OF SYSTEMATICS AND EVOLUTION
	2011-09-01
卷号	49 期号:5 页码:411-424
关键词	Dna Barcoding Ericaceae Gaultheria Species Discrimination Taxonomy Vaccinioideae
ISSN号	1674-4918
DOI	10.1111/j.1759-6831.2011.00152.x
通讯作者	wanghong@mail.kib.ac.cn
文献子类	Article
英文摘要	Four DNA barcoding loci, chloroplast loci rbcL, matK, trnH-psbA, and nuclear locus internal transcribed spacer (ITS), were tested for the accurate discrimination of the Chinese species of Gaultheria by using intraspecific and interspecific pairwise P-distance, Wilcoxon signed rank test, and tree-based analyses. This study included 186 individuals from 89 populations representing 30 species. For all individuals, single locus markers showed high levels of sequencing universality but were ineffective for species resolvability. Polymerase chain reaction amplification and sequencing were successful for all four loci. Both ITS and matK showed significantly higher levels of interspecific species delimitation than rbcL and trnH-psbA. A combination of matK and ITS was the most efficient DNA barcode among all studied regions, however, they do not represent an appropriate candidate barcode for Chinese Gaultheria, by which only 11 out of 30 species can be separated. Loci rbcL, matK, and trnH-psbA, which were recently proposed as universal plant barcodes, have a very poor capacity for species separation for Chinese Gaultheria. DNA barcodes may be reliable tools to identify the evolutionary units of this group, so further studies are needed to develop more efficient DNA barcodes for Gaultheria and other genera with complicated evolutionary histories.
学科主题	Plant Sciences
WOS关键词	LAND PLANTS ; EVOLUTION ; SEQUENCE ; REGION ; TAXA ; RBCL
WOS研究方向	Plant Sciences
语种	英语
WOS记录号	WOS:000295322200005
资助机构	Chinese Academy of Sciences[2009-LSF-GBOWS-01]; Western Light Talent Culture Project[2010312D11034]
公开日期	2012-03-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/3976]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Ren, He,Lu, Lu,Wang, Hong,et al. DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(5):411-424.
APA	Ren, He,Lu, Lu,Wang, Hong,&Li, De-Zhu.(2011).DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae).JOURNAL OF SYSTEMATICS AND EVOLUTION,49(5),411-424.
MLA	Ren, He,et al."DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.5(2011):411-424.