Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages

doi:10.1021/np9001465

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	Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages
	Aquila, Silvia 2; Weng, Zhi-Ying 3; Zeng, Yue-Qin 3; Sun, Han-Dong1 ; Luis Rios, Jose 2
刊名	JOURNAL OF NATURAL PRODUCTS
	2009-07-01
卷号	72 期号:7 页码:1269-1272
关键词	Trijugin-type Limonoids Trichilia-connaroides Sandoricum-koetjape Carbon Skeleton Meliaceae Baccifera Fruticosa
ISSN号	0163-3864
DOI	10.1021/np9001465
文献子类	Article
英文摘要	Xerophilusin A (1), xerophilusin B (2), longikaurin B (3), and xerophilusin F (4) from Isodon xerophylus inhibit LPS-induced NO production in RAW 264.7 macrophages, with IC(50) values of 0.60, 0.23, 0.44, and 0.67 mu M, respectively. and they all inhibited mRNA production in these same cells. They decreased the luciferase activity in RAW 264.7 cells transiently transfected with the NF-kappa B-dependent luciferase reporter, with IC(50) values of 1.8, 0.7. 1.2, and 1.6 mu M, respectively. Compounds 1-3 reduced NF-kappa B activation, with compound 4 showing no effect, but p65 translocation from the cytoplasm to the nucleus and the LPS-induced degradation of 1 kappa B were inhibited by all four test Compounds. These findings indicate that ent-kauranes are potential anti-inflammatory agents, with a specific mechanism in which both the inhibition of NF-kappa B translocation and the consequent decrease of pro-inflammatory mediators are implicated.
学科主题	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS关键词	ISODON-XEROPHILUS ; NO PRODUCTION ; NITRIC-OXIDE ; PATHWAY ; BINDING ; LEAVES ; CELLS
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy
语种	英语
WOS记录号	WOS:000268664500009
资助机构	University of Valencia ; Spanish Government [SAF2006-06726, NS-FC30772637]
公开日期	2012-03-21
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/4790]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 2.Univ Valencia, Fac Farm, Dept Farmacol, Valencia 46100, Spain 3.Kunming Med Coll, Dept Pharm, Kunming 650031, Peoples R China
推荐引用方式 GB/T 7714	Aquila, Silvia,Weng, Zhi-Ying,Zeng, Yue-Qin,et al. Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages[J]. JOURNAL OF NATURAL PRODUCTS,2009,72(7):1269-1272.
APA	Aquila, Silvia,Weng, Zhi-Ying,Zeng, Yue-Qin,Sun, Han-Dong,&Luis Rios, Jose.(2009).Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages.JOURNAL OF NATURAL PRODUCTS,72(7),1269-1272.
MLA	Aquila, Silvia,et al."Inhibition of NF-kappa B Activation and iNOS Induction by ent-Kaurane Diterpenoids in LPS-Stimulated RAW264.7 Murine Macrophages".JOURNAL OF NATURAL PRODUCTS 72.7(2009):1269-1272.