A geraniol-synthase gene from Cinnamomum tenuipilum

doi:10.1016/j.phytochem.2004.12.004

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	A geraniol-synthase gene from Cinnamomum tenuipilum
	Yang, T ; Li, J; Wang, HX ; Zeng, Y
刊名	PHYTOCHEMISTRY
	2005-02-01
卷号	66 期号:3 页码:285-293
关键词	Cinnamomum Tenuipilum Lauraceae Camphor Tree Functional Expression Chemotypes Essential Oil Ctges Gene Geraniol Synthase Monoterpene Synthases
ISSN号	0031-9422
DOI	10.1016/j.phytochem.2004.12.004
文献子类	Article
英文摘要	Geraniol may accumulate up to 86-98% of the leaf essential oils in geraniol chemotypes of the evergreen camphor tree Cinnamomum tenuipilum. A similarity-based cloning strategy yielded a cDNA clone that appeared to encode a terpene synthase and which could be phylogenetically grouped within the angiosperm monoterpene synthase/subfamily. After its expression in Escherichia coli and enzyme assay with prenyl diphosphates as substrates, the enzyme encoded by the putative C. tenuipilum monoterpene synthase gene was shown to specifically convert geranyl diphosphate to geraniol as a single product by GC-MS analysis. Biochemical characterization of the partially purified recombinant protein revealed a strong dependency for Mg2+ and Mn2+, and an apparent Michaelis constant of 55.8 muM for geranyl diphosphate. Thus, a new member of the monoterpene synthase family was identified and designated as CtGES. The genome contains a single copy of CtGES gene. Expression of CtGES was exclusively observed in the geraniol chemotype of C. tenuipilum. Furthermore, in situ hybridization analysis demonstrated that CtGES mRNA was localized in the oil cells of the leaves. (C) 2004 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
WOS关键词	LIMONENE SYNTHASE ; MYRCENE SYNTHASE ; SECRETORY-CELLS ; EXPRESSION ; L. ; BIOSYNTHESIS ; PEPPERMINT ; SUBFAMILY ; CLONING
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
WOS记录号	WOS:000227113100003
公开日期	2012-03-21
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/4722]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yang, T,Li, J,Wang, HX,et al. A geraniol-synthase gene from Cinnamomum tenuipilum[J]. PHYTOCHEMISTRY,2005,66(3):285-293.
APA	Yang, T,Li, J,Wang, HX,&Zeng, Y.(2005).A geraniol-synthase gene from Cinnamomum tenuipilum.PHYTOCHEMISTRY,66(3),285-293.
MLA	Yang, T,et al."A geraniol-synthase gene from Cinnamomum tenuipilum".PHYTOCHEMISTRY 66.3(2005):285-293.