alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase

doi:10.1371/journal.pone.0033376

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase
	Quan, Xiaofang 1; Wang, Yi 1; Ma, Xiaofeng 1; Liang, Yan 1; Tian, Weixi 1; Ma, Qingyun 2; Jiang, Hezhong 3; Zhao, Youxing 2
刊名	PLOS ONE
	2012-03-09
卷号	7 期号:3 页码:e33376
关键词	Aconitum Transsectum Diterpenoid Alkaloids Aconitramines d And e Norditerpenoid Alkaloids
ISSN号	1932-6203
DOI	10.1371/journal.pone.0033376
通讯作者	Quan, XF (reprint author), Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing, Peoples R China, maxiaofeng@gucas.ac.cn ; zhaoyx1011@163.com
文献子类	Article
英文摘要	alpha-Mangostin, isolated from the hulls of Garcinia mangostana L., was found to have in vitro cytotoxicity against 3T3-L1 cells as well as inhibiting fatty acid synthase (FAS, EC 2.3.1.85). Our studies showed that the cytotoxicity of alpha-mangostin with IC50 value of 20 mu M was incomplicated in apoptotic events including increase of cell membrane permeability, nuclear chromatin condensation and mitochondrial membrane potential (Delta Psi m) loss. This cytotoxicity was accompanied by the reduction of FAS activity in cells and could be rescued by 50 mM or 100 mM exogenous palmitic acids, which suggested that the apoptosis of 3T3-L1 preadipocytes induced by alpha-mangostin was via inhibition of FAS. Futhermore, alpha-mangostin could suppress intracellular lipid accumulation in the differentiating adipocytes and stimulated lipolysis in mature adipocytes, which was also related to its inhibition of FAS. In addition, 3T3-L1 preadipocytes were more susceptible to the cytotoxic effect of alpha-mangostin than mature adipocytes. Further studies showed that alpha-mangostin inhibited FAS probably by stronger action on the ketoacyl synthase domain and weaker action on the acetyl/malonyl transferase domain. These findings suggested that alpha-mangostin might be useful for preventing or treating obesity.
学科主题	Biology
WOS关键词	HYPOTHALAMIC MALONYL-COA ; NF-KAPPA-B ; GARCINIA-MANGOSTANA ; ADIPOSE-TISSUE ; ADIPOCYTE DIFFERENTIATION ; SIGNALING PATHWAY ; FOOD-INTAKE ; OBESITY ; SYNTHETASE ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000303062800054
资助机构	Special Science and Technology Projects for Outstanding Young in Life Sciences[KSCX2-EW-Q-19]; Twelfth Five Year Plan Basic Frontier Project in Life Sciences[Y129015EA2]; Natural Science Foundation of Yunnan[2008CD159]; GUCAS[O95101PY00]; K.C. Wong Education Foundation, Hong Kong
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/10417]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Quan, Xiaofang,Wang, Yi,Ma, Xiaofeng,et al. alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase[J]. PLOS ONE,2012,7(3):e33376.
APA	Quan, Xiaofang.,Wang, Yi.,Ma, Xiaofeng.,Liang, Yan.,Tian, Weixi.,...&Zhao, Youxing.(2012).alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase.PLOS ONE,7(3),e33376.
MLA	Quan, Xiaofang,et al."alpha-Mangostin Induces Apoptosis and Suppresses Differentiation of 3T3-L1 Cells via Inhibiting Fatty Acid Synthase".PLOS ONE 7.3(2012):e33376.