Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris

doi:10.1016/j.chroma.2012.01.008

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris
	Hou, Yuanyuan 2,3,4; Cao, Xuelin 5; Dong, Linyi 5; Wang, Liqiang 2; Cheng, Binfeng 2; Shi, Qian 2; Luo, Xiaodong1 ; Bai, Gang 2,3,4
刊名	JOURNAL OF CHROMATOGRAPHY A
	2012-03-02
卷号	1227 页码:203-209
关键词	Bioactivity-integrated Fingerprinting Constituent Identification Beta(2) Adrenergic Receptor Agonist Lcms-it-tof
ISSN号	0021-9673
DOI	10.1016/j.chroma.2012.01.008
通讯作者	Luo, XD (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China, xdluo@kib.ac.cn.cn ; gangbai@nankai.edu.cn
文献子类	Article
英文摘要	Although chromatographic fingerprinting combined with chemometrics, is a rational method for the quality control of traditional Chinese medicine (TCM), chemometrics cannot fully explore the relationship between chemical information and the efficacy of the potential activity. In the present work, a cell-based beta(2) adrenergic receptor (beta(2)AR) agonist functional evaluation model coupled with high-performance liquid chromatography was developed to screen the potential beta(2)AR agonist components in the alkaloidal extract of Alstonia scholaris leaves. Using a liquid chromatography with ion trap time-of-flight mass spectrometry (LCMS-IT-TOF) system, the potential bioactive compounds in the prescription were identified and deduced based on the mass spectrometric fragmentation patterns, tandem mass spectrometry (MS/MS) data, and relevant literature. Several new beta(2)AR agonists of indole alkaloids were successfully found, and their activities were confirmed through an in vivo relaxant test on guinea pig tracheal muscles. The developed method is rapid and reliable compared with conventional fingerprinting and showed high sensitivity and resolution for the identification of beta(2)AR agonists in TCM prescriptions. This strategy clearly demonstrates that bioactivity-integrated fingerprinting is a powerful tool not only in screening and identifying potential lead compounds and in determining the therapeutic material basis of Chinese herbal prescriptions, but also in supplying suitable chemical markers for their quality control. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods ; Chemistry, Analytical
WOS关键词	TRADITIONAL CHINESE MEDICINES ; BIOLOGICAL FINGERPRINTING ANALYSIS ; LONGDAN-XIEGAN-DECOCTION ; COMBINED PRESCRIPTION ; ACTIVE COMPONENTS ; INDOLE ALKALOIDS ; CELL EXTRACTION ; QUALITY-CONTROL ; PROTEIN ; SYSTEM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
WOS记录号	WOS:000301563700023
资助机构	National Natural Science Foundation of China[81173638, 81001682]; Fundamental Research Funds for the Central Universities[65011111]
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/10377]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China 2.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 3.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China 4.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China 5.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
推荐引用方式 GB/T 7714	Hou, Yuanyuan,Cao, Xuelin,Dong, Linyi,et al. Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1227:203-209.
APA	Hou, Yuanyuan.,Cao, Xuelin.,Dong, Linyi.,Wang, Liqiang.,Cheng, Binfeng.,...&Bai, Gang.(2012).Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY A,1227,203-209.
MLA	Hou, Yuanyuan,et al."Bioactivity-based liquid chromatography-coupled electrospray ionization tandem ion trap/time of flight mass spectrometry for beta(2)AR agonist identification in alkaloidal extract of Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY A 1227(2012):203-209.