Identification and evaluation of apoptotic compounds from Garcinia oligantha

doi:10.1016/j.bmcl.2012.01.068

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Identification and evaluation of apoptotic compounds from Garcinia oligantha
	Gao, Xue-Mei 1,3; Yu, Ting 2; Cui, Ming-zhu 1,3; Pu, Jian-Xin4 ; Du, Xue 4; Han, Quan-bin 5; Hu, Qiu-fen 1,3; Liu, Tian-Cheng 1,3; Luo, Kathy Qian 2; Xu, Hong-Xi 6
刊名	BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
	2012-03-15
卷号	22 期号:6 页码:2350-2353
关键词	Anticancer Drug Garcinia Oligantha Xanthone Apoptosis Caspase-3
ISSN号	0960-894X
DOI	10.1016/j.bmcl.2012.01.068
通讯作者	Gao, XM (reprint author), Yunnan Univ Nationalities, Key Lab Ethn Med Resource Chem, State Ethn Affairs Commiss, 134 Yi Er Yi Ave, Kunming 650031, Yunnan, Peoples R China, gao_xuemei@hotmail.com ; kluo@ntu.edu.sg ; xuhongxi88@gmail.com
文献子类	Article
英文摘要	Four new compounds, oliganthins A-D (1-4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 mu M or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 mu M, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 mu M, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Medicinal ; Chemistry, Organic
WOS关键词	DERIVATIVES ; ACTIVATION
WOS研究方向	Pharmacology & Pharmacy ; Chemistry
语种	英语
WOS记录号	WOS:000301285500047
资助机构	National Natural Science Foundation of China[21002085, 81073014, 81173485]; Key Laboratory of Ethnic Medicine Resource Chemistry (Yunnan University of Nationalities); State Ethnic Affairs Commission & Ministry of Education[MZY100105]; Yunnan University of Nationalities; Minister of Education in Singapore
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/10365]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Yunnan Univ Nationalities, Key Lab Ethn Med Resource Chem, State Ethn Affairs Commiss, Kunming 650031, Yunnan, Peoples R China 2.Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore 3.Yunnan Univ Nationalities, Minist Educ, Kunming 650031, Yunnan, Peoples R China 4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 5.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China 6.Shanghai Univ Tradit Chinese Med Cai Lun Lu 1200, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Gao, Xue-Mei,Yu, Ting,Cui, Ming-zhu,et al. Identification and evaluation of apoptotic compounds from Garcinia oligantha[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2012,22(6):2350-2353.
APA	Gao, Xue-Mei.,Yu, Ting.,Cui, Ming-zhu.,Pu, Jian-Xin.,Du, Xue.,...&Xu, Hong-Xi.(2012).Identification and evaluation of apoptotic compounds from Garcinia oligantha.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,22(6),2350-2353.
MLA	Gao, Xue-Mei,et al."Identification and evaluation of apoptotic compounds from Garcinia oligantha".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 22.6(2012):2350-2353.