A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)

doi:10.1016/j.talanta.2015.09.014

	A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)
	Zhang, JC; Chen, J; Zhang, HT; Hu Y(胡毅); Chai ZF(柴之芳); Zhao YL(赵宇亮); Chen J(陈俊); Lu, HR; Lu HR(卢会茹); Hu, Y
刊名	TALANTA
	2016
卷号	146 页码:477
关键词	Copper Cysteine Fluorescent probe Ellman's reagent Cell imaging
DOI	10.1016/j.talanta.2015.09.014
通讯作者	胡毅
英文摘要	In this study, Cu(II)-mediated differential alteration of cysteine (Cys) reactivity is reported by using a Cys-specific fluorescent probe. The probe could react with Cys to give out strong fluorescence. When Cys was preincubated with Cu(II), the fluorescence of the probe was decreased due to the inhibition of Cys's reactivity by Cu(II). Remarkably, experimental results reveal that the probe could detect Cu(II) at subnanomolar concentrations. In contrast, Cu(II) could only partially inhibit the reaction between Cys and Ellman's reagent (DTNB). Furthermore, selectivity experiments show that Cu(II) is a much more potent inhibitor for Cys compared to other metal ions. Cell imaging experiments also confirm the inhibitory effects of Cu(II) on Cys's reactivity in living cells. We envision that the probe could add a useful tool for sensitive and selective detection of Cu(II) for biomedical research. (C) 2015 Elsevier B.V. All rights reserved.
语种	英语
WOS记录号	WOS:000363815600068
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/261058]
专题	高能物理研究所_院士高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Zhang, JC,Chen, J,Zhang, HT,et al. A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)[J]. TALANTA,2016,146:477.
APA	Zhang, JC.,Chen, J.,Zhang, HT.,胡毅.,柴之芳.,...&Liu, RC.(2016).A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II).TALANTA,146,477.
MLA	Zhang, JC,et al."A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)".TALANTA 146(2016):477.