Measurement of protein size in concentrated solutions by small angle X-ray scattering

doi:10.1002/pro.2957

	Measurement of protein size in concentrated solutions by small angle X-ray scattering
	Liu, J; Liu J(刘俊); Li ZH(李志宏); Wei YR(魏彦茹); Wang WJ(王文佳); Wang B(汪冰); Liang HL(梁红丽); Gao YX(高愈希); Li, ZH; Wei, YR
刊名	PROTEIN SCIENCE
	2016
卷号	25 期号:8 页码:1385-1389
关键词	protein size inter-particle interference distance distribution function small angle X-ray scattering
DOI	10.1002/pro.2957
通讯作者	李志宏
英文摘要	By simulations on the distance distribution function (DDF) derived from small angle X-ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.
语种	英语
WOS记录号	WOS:000380068700003
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/260455]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Liu, J,Liu J,Li ZH,et al. Measurement of protein size in concentrated solutions by small angle X-ray scattering[J]. PROTEIN SCIENCE,2016,25(8):1385-1389.
APA	Liu, J.,刘俊.,李志宏.,魏彦茹.,王文佳.,...&Gao, YX.(2016).Measurement of protein size in concentrated solutions by small angle X-ray scattering.PROTEIN SCIENCE,25(8),1385-1389.
MLA	Liu, J,et al."Measurement of protein size in concentrated solutions by small angle X-ray scattering".PROTEIN SCIENCE 25.8(2016):1385-1389.