Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation

doi:10.1186/s11671-016-1487-5

	Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation
	Chen K(陈奎); Zhang MY(张铭倚); Chang YN(常亚男); Xia L(夏林); Gu WH(古伟宏); Qin YX(秦艳霞); Xing GM(邢更妹); Chen, K; Zhang, MY; Chang, YN
刊名	NANOSCALE RESEARCH LETTERS
	2016
卷号	11 期号:1 页码:304
关键词	DNA methylation Gold nanoparticles probe Surface plasmon resonance Visual detection Semi-quantitative assay
DOI	10.1186/s11671-016-1487-5
通讯作者	邢更妹
文献子类	期刊论文
英文摘要	The surface plasmon resonance (SPR) effect endows gold nanoparticles (GNPs) with the ability to visualize biomolecules. In the present study, we designed and constructed a GNP probe to allow the semi-quantitative analysis of methylated tumor suppressor genes in cultured cells. To construct the probe, the GNP surfaces were coated with single-stranded DNA (ssDNA) by forming Au-S bonds. The ssDNA contains a thiolated 5'-end, a regulatory domain of 12 adenine nucleotides, and a functional domain with absolute pairing with methylated p16 sequence (Met-p16). The probe, paired with Met-p16, clearly changed the color of aggregating GNPs probe in 5 mol/L NaCl solution. Utilizing the probe, p16 gene methylation in HCT116 cells was semi-quantified. Further, the methylation of E-cadherin, p15, and p16 gene in Caco2, HepG2, and HCT116 cell lines were detected by the corresponding probes, constructed with three domains. This simple and cost-effective method was useful for the diagnosis of DNA methylation-related diseases.
WOS关键词	COLORIMETRIC DETECTION ; HUMAN CANCERS ; ASSAY ; GENE ; AMPLIFICATION ; HYBRIDIZATION ; INACTIVATION ; AGGREGATION ; CELLS ; SIZE
语种	英语
WOS记录号	WOS:000391788400001
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/260275]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Chen K,Zhang MY,Chang YN,et al. Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation[J]. NANOSCALE RESEARCH LETTERS,2016,11(1):304.
APA	陈奎.,张铭倚.,常亚男.,夏林.,古伟宏.,...&Xing, GM.(2016).Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation.NANOSCALE RESEARCH LETTERS,11(1),304.
MLA	陈奎,et al."Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation".NANOSCALE RESEARCH LETTERS 11.1(2016):304.