The Genomes of Oryza sativa: A history of duplications

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	The Genomes of Oryza sativa: A history of duplications
	Wang, JQ; Li, DW; Liu, DY; Zhang, XW; Ji, ZD; Zhao, WM; Sun, YQ; Zhang, ZP; Bao, JY; Han, YJ
刊名	PLOS BIOLOGY
	2005
卷号	3 期号:2 页码:266-281
关键词	Rice Genome Arabidopsis-thaliana Sequence-analysis Protein Networks Draft Sequence Plant Genomes Genes Evolution Dna Annotation
ISSN号	1544-9173
英文摘要	We report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. Tested against a nonredundant collection of 19,079 full-length cDNAs, 97.7% of the genes are aligned, without fragmentation, to the mapped superscaffolds of one or the other genome. We introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous predictions resulting from transposable elements. Using the available EST data to adjust for residual errors in the predictions, the estimated gene count is at least 38,000 - 40,000. Only 2% - 3% of the genes are unique to any one subspecies, comparable to the amount of sequence that might still be missing. Despite this lack of variation in gene content, there is enormous variation in the intergenic regions. At least a quarter of the two sequences could not be aligned, and where they could be aligned, single nucleotide polymorphism ( SNP) rates varied from as little as 3.0 SNP/kb in the coding regions to 27.6 SNP/kb in the transposable elements. A more inclusive new approach for analyzing duplication history is introduced here. It reveals an ancient whole-genome duplication, a recent segmental duplication on Chromosomes 11 and 12, and massive ongoing individual gene duplications. We find 18 distinct pairs of duplicated segments that cover 65.7% of the genome; 17 of these pairs date back to a common time before the divergence of the grasses. More important, ongoing individual gene duplications provide a never-ending source of raw material for gene genesis and are major contributors to the differences between members of the grass family.
学科主题	Physics
URL标识	查看原文
WOS记录号	WOS:000227404100011
公开日期	2012-08-30
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/13892]
专题	理论物理研究所_理论物理所1978-2010年知识产出
通讯作者	Yu, J , Chinese Acad Sci, Beijing Genom Inst, Beijing Proteom Inst, Beijing Inst Genom, Beijing, Peoples R China.
推荐引用方式 GB/T 7714	Wang, JQ,Li, DW,Liu, DY,et al. The Genomes of Oryza sativa: A history of duplications[J]. PLOS BIOLOGY,2005,3(2):266-281.
APA	Wang, JQ.,Li, DW.,Liu, DY.,Zhang, XW.,Ji, ZD.,...&Ren, XY.(2005).The Genomes of Oryza sativa: A history of duplications.PLOS BIOLOGY,3(2),266-281.
MLA	Wang, JQ,et al."The Genomes of Oryza sativa: A history of duplications".PLOS BIOLOGY 3.2(2005):266-281.