Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS

	Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS
	You, Jinmao ; Zhao, Xianen ; Suo, Yourui ; Li, Yulin ; Wang, Honglun ; Chen, Guichen
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2007-04-01
卷号	848 期号:2 页码:283-291
关键词	1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) derivatization HPLC fatty acids mass spectrometry
合作状况	其它
中文摘要	a sensitive method for the determination of long-chain fatty acids (lcfas) (>c20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4.5-f]-9,10-phenanthrene (tspp) as tagging reagent with fluorescence detection and identification with post-column apci/ms has been developed. the lcfas in bryophyte plant samples were obtained based on distillation extraction with 1: 1 (v/v) chloroform/methanol as extracting solvent. tspp could easily and quickly label lcfas at 90 degrees c in the presence of k2co3 catalyst in dmf. eleven free lcfas from the extracts of bryophyte plants were sensitively determined. maximal labeling yields close to 100% were observed with a five-fold excess of molar reagent. separation of the derivatized fatty acids exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase eclipse xdb-c-8 column. calculated detection limits from 1.0 pmol injection, at a signal-to-noise ratio of 3, were 26.19-76.67 fmol. excellent linear responses were observed with coefficients of >0.9996. good compositional data were obtained from the analysis of the extracted lcfas containing as little as 0.2 g of bryophyte plant samples. therefore, the facile tspp derivatization coupled with hplc/apci/ms analysis allowed the development of a highly sensitive method for the quantitation of trace levels of lcfas from biological and natural environmental samples. (c) 2006 elsevier b.v. all rights reserved.
英文摘要	a sensitive method for the determination of long-chain fatty acids (lcfas) (>c20) using 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4.5-f]-9,10-phenanthrene (tspp) as tagging reagent with fluorescence detection and identification with post-column apci/ms has been developed. the lcfas in bryophyte plant samples were obtained based on distillation extraction with 1: 1 (v/v) chloroform/methanol as extracting solvent. tspp could easily and quickly label lcfas at 90 degrees c in the presence of k2co3 catalyst in dmf. eleven free lcfas from the extracts of bryophyte plants were sensitively determined. maximal labeling yields close to 100% were observed with a five-fold excess of molar reagent. separation of the derivatized fatty acids exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase eclipse xdb-c-8 column. calculated detection limits from 1.0 pmol injection, at a signal-to-noise ratio of 3, were 26.19-76.67 fmol. excellent linear responses were observed with coefficients of >0.9996. good compositional data were obtained from the analysis of the extracted lcfas containing as little as 0.2 g of bryophyte plant samples. therefore, the facile tspp derivatization coupled with hplc/apci/ms analysis allowed the development of a highly sensitive method for the quantitation of trace levels of lcfas from biological and natural environmental samples. (c) 2006 elsevier b.v. all rights reserved.
学科主题	生物化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; carboxylic-acids ; derivatization reagent ; labeling reagent ; precolumn derivatization ; fluorometric detection ; derivatives ; liverworts ; japanese ; serum
收录类别	SCI
语种	英语
WOS记录号	WOS:000245762600016
公开日期	2009-12-07
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1282]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Shandong 273165, Peoples R China
推荐引用方式 GB/T 7714	You, Jinmao,Zhao, Xianen,Suo, Yourui,et al. Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2007,848(2):283-291.
APA	You, Jinmao,Zhao, Xianen,Suo, Yourui,Li, Yulin,Wang, Honglun,&Chen, Guichen.(2007).Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS.journal of chromatography b-analytical technologies in the biomedical and life sciences,848(2),283-291.
MLA	You, Jinmao,et al."Determination of long-chain fatty acids in bryophyte plants extracts by HPLC with fluorescence detection and identification with MS".journal of chromatography b-analytical technologies in the biomedical and life sciences 848.2(2007):283-291.