Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters
	Xu, Xinning ; Dang D(党丹); Xi N(席宁); Wang YC(王越超); Li M(李密); Liu LQ(刘连庆); Xing, Xiaojing
刊名	Journal of the Mechanical Behavior of Biomedical Materials
	2018
卷号	82 页码:193-201
关键词	Atomic force microscopy Cell mechanics Viscoelasticity Primary cell Relaxation time
ISSN号	1751-6161
通讯作者	Li M(李密) ; Liu LQ(刘连庆)
产权排序	1
中文摘要	Cell mechanics plays an important role in regulating the physiological activities of cells. The advent of atomic force microscopy (AFM) provides a novel powerful instrument for quantifying the mechanics of single cells at the nanoscale. The applications of AFM in single-cell mechanical assays in the past decade have significantly contributed to the field of cell and molecular biology. However, current AFM-based cellular mechanical studies are commonly carried out with fixed measurement parameters, which provides limited information about the dynamic cellular mechanical behaviors in response to the variable external stimuli. In this work, we utilized AFM to investigate cellular viscoelasticity (portrayed as relaxation time) with varying measurement parameters, including ramp rate and surface dwell time, on both cell lines and primary cells. The experimental results show that the obtained cellular relaxation times are remarkably dependent on the parameter surface dwell time and ramp rate during measurements. Besides, the dependencies to the measurement parameters are variable for different types of cells, which can be potentially used to indicate cell states. The research improves our understanding of single-cell dynamic rheology and provides a novel idea for discriminating different types of cells by AFM-based cellular viscoelastic assays with varying measurement parameters.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	BREAST-CANCER CELLS ; LIVING CELLS ; SINGLE-CELL ; MECHANICS ; BIOLOGY ; AFM ; MICROENVIRONMENT ; MICRORHEOLOGY ; GENERATION ; RHEOLOGY
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000432508800023
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/21732]
专题	沈阳自动化研究所_机器人学研究室
作者单位	1.School of Medical Devices, Shenyang Pharmaceutical University, Shenyang 110016, China 2.Department of Hematology, Liaoning Cancer Hospital, Shenyang 110042, China 3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 4.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong
推荐引用方式 GB/T 7714	Xu, Xinning,Dang D,Xi N,et al. Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters[J]. Journal of the Mechanical Behavior of Biomedical Materials,2018,82:193-201.
APA	Xu, Xinning.,Dang D.,Xi N.,Wang YC.,Li M.,...&Xing, Xiaojing.(2018).Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters.Journal of the Mechanical Behavior of Biomedical Materials,82,193-201.
MLA	Xu, Xinning,et al."Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters".Journal of the Mechanical Behavior of Biomedical Materials 82(2018):193-201.