Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods

doi:10.1111/j.1462-2920.2010.02220.x

	Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods
	Jogler, C.1; Niebler, M.1; Lin, W.2; Kube, M.3; Wanner, G.1; Kolinko, S.1; Stief, P.4; Beck, A. J.4; de Beer, D.4; Petersen, N.5
刊名	ENVIRONMENTAL MICROBIOLOGY
	2010-09-01
卷号	12 期号:9 页码:2466-2478
ISSN号	1462-2912
DOI	10.1111/j.1462-2920.2010.02220.x
文献子类	Article
英文摘要	P>'Candidatus Magnetobacterium bavaricum' is unusual among magnetotactic bacteria (MTB) in terms of cell size (8-10 mu m long, 1.5-2 mu m in diameter), cell architecture, magnetotactic behaviour and its distinct phylogenetic position in the deep-branching Nitrospira phylum. In the present study, improved magnetic enrichment techniques permitted high-resolution scanning electron microscopy and energy dispersive X-ray analysis, which revealed the intracellular organization of the magnetosome chains. Sulfur globule accumulation in the cytoplasm point towards a sulfur-oxidizing metabolism of 'Candidatus M. bavaricum'. Detailed analysis of 'Candidatus M. bavaricum' microhabitats revealed more complex distribution patterns than previously reported, with cells predominantly found in low oxygen concentration. No correlation to other geochemical parameters could be observed. In addition, the analysis of a metagenomic fosmid library revealed a 34 kb genomic fragment, which contains 33 genes, among them the complete rRNA gene operon of 'Candidatus M. bavaricum' as well as a gene encoding a putative type IV RubisCO large subunit.
WOS关键词	UNCULTIVATED MAGNETOTACTIC BACTERIA ; WATER TREATMENT PLANTS ; RUBISCO-LIKE PROTEINS ; SP STRAIN AMB-1 ; MAGNETOSOME ISLAND ; SULFUR METABOLISM ; GENE CLUSTERS ; SEDIMENTS ; DIVERSITY ; IDENTIFICATION
WOS研究方向	Microbiology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000281556900007
资助机构	German Research Foundation (DFG) ; German Research Foundation (DFG) ; Max Planck Society (MPG) ; Max Planck Society (MPG) ; German Research Foundation (DFG) ; German Research Foundation (DFG) ; Max Planck Society (MPG) ; Max Planck Society (MPG) ; German Research Foundation (DFG) ; German Research Foundation (DFG) ; Max Planck Society (MPG) ; Max Planck Society (MPG) ; German Research Foundation (DFG) ; German Research Foundation (DFG) ; Max Planck Society (MPG) ; Max Planck Society (MPG)
内容类型	期刊论文
源URL	[http://ir.iggcas.ac.cn/handle/132A11/73060]
专题	中国科学院地质与地球物理研究所
通讯作者	Schueller, D.
作者单位	1.LMU, Dept Biol 1, D-82152 Planegg Martinsried, Germany 2.Chinese Acad Sci, Paleomagnetism & Geochronol Lab, State Key Lab Lithospher Evolut, Inst Geol & Geophys,Biogeomagnetism Grp, Beijing 100029, Peoples R China 3.Max Planck Inst Mol Genet, D-14195 Berlin, Germany 4.Max Planck Inst Marine Microbiol, D-28539 Bremen, Germany 5.LMU, Dept Earth & Environm Sci, D-80333 Munich, Germany
推荐引用方式 GB/T 7714	Jogler, C.,Niebler, M.,Lin, W.,et al. Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods[J]. ENVIRONMENTAL MICROBIOLOGY,2010,12(9):2466-2478.
APA	Jogler, C..,Niebler, M..,Lin, W..,Kube, M..,Wanner, G..,...&Schueller, D..(2010).Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods.ENVIRONMENTAL MICROBIOLOGY,12(9),2466-2478.
MLA	Jogler, C.,et al."Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods".ENVIRONMENTAL MICROBIOLOGY 12.9(2010):2466-2478.