Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression

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	Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression
	Wang, Kang 2; Wang, Yanling 2; Gao, Lixin 2; Li, Xinmin 2; Li, Mingming 2; Guo, Jianyou 1; J. Y. Guo
刊名	BIOLOGICAL & PHARMACEUTICAL BULLETIN
	2008-08-01
卷号	31 期号:8 页码:1541-1546
关键词	dexamethasone vascular endothelial growth factor intercellular adhesion molecule-1 diabetes
ISSN号	0918-6158
文献子类	Article
英文摘要	Intravitreal injection of corticosteroid has been used to treat diabetic macular edema, however, the exact mechanism remains unknown. In the present experiment, four weeks after streptozotocin administration, intravitreal injection of dexamethasone (50 mu g/10 mu l) was performed. After 2 d injection, we investigated the effect of dexamethasone on leukocyte accumulation, vascular permeability and the expression of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in streptozotocin-diabetic rats. Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, the retinal vascular permeability was measured by the Evans blue assay. The mRNA and protein level of VEGF and ICAM-1 were analyzed with real-time quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. Dexamethasone downregulated VEGF and ICAM-1 expression in diabetic rats which correlated with its effect on leukocytes accumulation and retinal vascular permeability. The present data revealed that dexamethasone may inhibit retinal accumulation and leukostasis accumulation and vascular permeability through its blockage on VEGF and ICAM-1 expression.; Intravitreal injection of corticosteroid has been used to treat diabetic macular edema, however, the exact mechanism remains unknown. In the present experiment, four weeks after streptozotocin administration, intravitreal injection of dexamethasone (50 mu g/10 mu l) was performed. After 2 d injection, we investigated the effect of dexamethasone on leukocyte accumulation, vascular permeability and the expression of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in streptozotocin-diabetic rats. Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, the retinal vascular permeability was measured by the Evans blue assay. The mRNA and protein level of VEGF and ICAM-1 were analyzed with real-time quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) respectively. Dexamethasone downregulated VEGF and ICAM-1 expression in diabetic rats which correlated with its effect on leukocytes accumulation and retinal vascular permeability. The present data revealed that dexamethasone may inhibit retinal accumulation and leukostasis accumulation and vascular permeability through its blockage on VEGF and ICAM-1 expression.
学科主题	生理心理学/生物心理学
语种	英语
WOS记录号	WOS:000259174000013
公开日期	2011-08-22
内容类型	期刊论文
源URL	[http://ir.psych.ac.cn/handle/311026/5312]
专题	心理研究所_中国科学院心理研究所回溯数据库(1956-2010)
通讯作者	J. Y. Guo
作者单位	1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Healthy, Beijing 100101, Peoples R China 2.Beijing Capital Med Univ, Affiliated Beijing Friendship Hosp, Dept Ophthalmol, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kang,Wang, Yanling,Gao, Lixin,et al. Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2008,31(8):1541-1546.
APA	Wang, Kang.,Wang, Yanling.,Gao, Lixin.,Li, Xinmin.,Li, Mingming.,...&J. Y. Guo.(2008).Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression.BIOLOGICAL & PHARMACEUTICAL BULLETIN,31(8),1541-1546.
MLA	Wang, Kang,et al."Dexamethasone inhibits leukocyte accumulation and vascular permeability in retina of streptozotocin-induced diabetic rats via reducing vascular endothelial growth factor and intercellular adhesion molecule-1 expression".BIOLOGICAL & PHARMACEUTICAL BULLETIN 31.8(2008):1541-1546.