Effects of stepwise nitrogen depletion on carotenoid content, fluorescence parameters and the cellular stoichiometry of Chlorella vulgaris

doi:10.1016/j.saa.2017.03.022

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 水质生物转化研究中心

	Effects of stepwise nitrogen depletion on carotenoid content, fluorescence parameters and the cellular stoichiometry of Chlorella vulgaris
	Zhang, Ping 1; Li, Zhe2 ; Lu, Lunhui2 ; Xiao, Yan2 ; Liu, Jing 1; Guo, Jinsong 1,2; Fang, Fang 1
刊名	SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
	2017-06-15
卷号	181 页码:30-38
关键词	Nitrogen stress Carotenoids Cellular stoichiometry Raman spectroscopy Chlorella vulgaris
ISSN号	1386-1425
DOI	10.1016/j.saa.2017.03.022
通讯作者	Li, Z (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China.
英文摘要	Stressful conditions can stimulate the accumulation of carotenoids in some microalgae. To obtain more knowledge of the stress response, we studied the effects of different N concentrations on unicellular content of carotenoids using Raman spectroscopic technique; cellular stoichiometric changes and the fluorescence parameters of Chlorella vulgaris were concomitantly studied. Initially, we optimized the Raman scattering conditions and demonstrated the feasibility of unicellular carotenoid analysis by Raman spectroscopic technique. The results showed that an integration time of 10 s, laser power at 0.1 mW and an accumulation time of 1 were the optimum conditions, and the peak height at 1523 cm(-1) scaled linearly with the carotenoid content in the range of 0.625-1440 mg/L with a recovery rate of 97% similar to 103%. In the experiment, seven different nitrogen levels ranging from 0 to 2.48 x 10(5) mu g/L were imposed. Samples were taken at the start, exponential phase and end of the experiment The results showed that nitrogen stress can facilitate the synthesis of carotenoids, while at the same time, excessive nitrogen stress led to lower proliferative and photosynthetic activity. Compared with carotenoids, chlorophylls were more sensitive to nitrogen stress; it declined dramatically as stress processed. There existed no significant differences for Fv/Fm among different nitrogen levels during the exponential phase, while in the end, it declined and a significant difference appeared between cells in 2.48 x 10(5) mu g/L N and other experimental levels. Photosynthetic efficiency, namely the C/N mole ratio in algal cells, didnot significantly change during the exponential phase; however, apparent increases ultimately occurred, except for the stable C/N in BG11 medium. This increase matched well with the carotenoid decline, indicating that an increasing cellular C/N mole ratio can be used as an indicator of excessive stress in carotenoid production. Besides, there also existed an inverse correlation with ETRmax. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[51679226] ; National Natural Science Foundation of China[41430750] ; Chongqing Research Program of Basic Research and Frontier Technology[cstc2015jcyjBX0006]
WOS研究方向	Spectroscopy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000401391700006
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/207]
专题	水质生物转化研究中心
通讯作者	Li, Zhe
作者单位	1.Chongqing Univ, Key Lab, Three Gorges Reservoir Regions Eco Environments M, Chongqing 400045, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ping,Li, Zhe,Lu, Lunhui,et al. Effects of stepwise nitrogen depletion on carotenoid content, fluorescence parameters and the cellular stoichiometry of Chlorella vulgaris[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2017,181:30-38.
APA	Zhang, Ping.,Li, Zhe.,Lu, Lunhui.,Xiao, Yan.,Liu, Jing.,...&Fang, Fang.(2017).Effects of stepwise nitrogen depletion on carotenoid content, fluorescence parameters and the cellular stoichiometry of Chlorella vulgaris.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,181,30-38.
MLA	Zhang, Ping,et al."Effects of stepwise nitrogen depletion on carotenoid content, fluorescence parameters and the cellular stoichiometry of Chlorella vulgaris".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 181(2017):30-38.