Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study

doi:10.1021/acsami.5b03041

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 太赫兹技术研究中心

	Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study
	Wang, Huabin1,3 ; Wilksch, Jonathan J.2; Strugnell, Richard A.2; Gee, Michelle L.1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2015-06-17
卷号	7 期号:23 页码:13007-13013
关键词	bacteria capsule polysaccharides fimbriae biofilm nanomechanics
ISSN号	1944-8244
DOI	10.1021/acsami.5b03041
通讯作者	Strugnell, RA (reprint author), Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3010, Australia.
英文摘要	Bacteria form biofilms to facilitate colonization of biotic and abiotic surfaces, and biofilm formation on indwelling medical devices is a common cause of hospital-acquired infection. Although it is well-recognized that the exopolysaccharide capsule is one of the key bacterial components for biofilm formation, the underlying biophysical mechanism is poorly understood. In the present study, nanomechanical measurements of wild type and specific mutants of the pathogen, Klebsiella pneumoniae, were performed in situ using atomic force microscopy (AFM). Theoretical modeling of the mechanical data and static microtiter plate biofilm assays show that the organization of the capsule can influence bacterial adhesion, and thereby biofilm formation. The capsular organization is affected by the presence of type 3 fimbriae. Understanding the biophysical mechanisms for the impact of the structural organization of the bacterial polysaccharide capsule on biofilm formation will aid the development of strategies to prevent biofilm formation.
资助项目	Melbourne Materials Institute ; Australian Government National Health and Medical Research Council (NHMRC)[628770] ; NHMRC[606788] ; Australian Research Council[DP130100957] ; NSFC[11105090] ; CIGIT for Startup Foundation for Advanced Talents ; Chongqing Science and Technology Commission[cstc2013yykfC00007] ; Chongqing Science and Technology Commission[cstc2013jcyjC00001] ; Chinese Academy of Sciences for a Key Scientific Instrument and Equipment Development Project
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000356753500080
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/1749]
专题	太赫兹技术研究中心
通讯作者	Strugnell, Richard A.
作者单位	1.Univ Melbourne, Dept Chem, Parkville, Vic 3010, Australia 2.Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3010, Australia 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Wang, Huabin,Wilksch, Jonathan J.,Strugnell, Richard A.,et al. Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(23):13007-13013.
APA	Wang, Huabin,Wilksch, Jonathan J.,Strugnell, Richard A.,&Gee, Michelle L..(2015).Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study.ACS APPLIED MATERIALS & INTERFACES,7(23),13007-13013.
MLA	Wang, Huabin,et al."Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study".ACS APPLIED MATERIALS & INTERFACES 7.23(2015):13007-13013.