Ultrasensitive colorimetric and fluorometric detection of Hg(II) based on the use of gold nanoparticles and a catalytic hairpin assembly

doi:10.1007/s00604-017-2516-5

CORC > 重庆绿色智能技术研究院 > 中国科学院重庆绿色智能技术研究院 > 太赫兹技术研究中心

	Ultrasensitive colorimetric and fluorometric detection of Hg(II) based on the use of gold nanoparticles and a catalytic hairpin assembly
	Yang, Lizhu 1; Yun, Wen 2; Chen, Yilin 3; Wu, Hong 2; Liu, Xingyan 2; Fu, Min 2; Huang, Yu4 ; Yang, Lizhu 5; Yun, Wen 6; Chen, Yilin 7
刊名	MICROCHIMICA ACTA
	2017-12-01
卷号	184 期号:12 页码:4741-4747
关键词	Hairpins Gold nanoparticles Signal amplification Mercury ions Colorimetry Fluorescence
ISSN号	0026-3672
DOI	10.1007/s00604-017-2516-5
通讯作者	Yun, W (reprint author), Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China. ; Huang, Y (reprint author), Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China.
英文摘要	An ultrasensitive method for colorimetric and fluorometric determination of Hg(II) is described. It involves a dual signal detection strategy based on the use of gold nano-particles (AuNPs) and of a catalytic hairpin self-assembly. Three fluorophore-labeled metastable hairpin DNA probes were adsorbed on AuNPs to quench the fluorescence of the labels. The addition of Hg(II) causes catalytic self-assembly of the hairpins due to formation of T-Hg(II)-T base pairs with the aid of helper DNA to form a branched junction. The branched junction is a dsDNA that is released from the surface of the AuNPs. As a result, fluorescence recovers. Its intensity, best measured at excitation/emission wavelengths of 492/517 nm, increases linearly in the 0.2 to 100 nM of Hg(II) concentration range. The limit of detection is 0.1 nM. This is comparable to, or better than in other amplification based methods. The detection scheme offers both colorimetric (visual) and fluorescent read-out.
资助项目	National Natural Science Foundation of China[31300819] ; Open Research Fund[CQCM-2016-05] ; Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences[201712] ; Chongqing Municipal Education Commission[KJ1706156] ; Wenzhou Science & Technology Bureau[S20150012] ; Key Laboratory of Reservoir Aquatic Environment of CAS
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000414547400021
内容类型	期刊论文
源URL	[http://172.16.51.4:88/handle/2HOD01W0/115]
专题	太赫兹技术研究中心
通讯作者	Yun, Wen; Huang, Yu; Yun, Wen; Huang, Yu
作者单位	1.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China 2.Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China 3.Chongqing Acad Metrol & Qual Inspect, Chongqing 401123, Peoples R China 4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China 5.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China 6.Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China 7.Chongqing Acad Metrol & Qual Inspect, Chongqing 401123, Peoples R China 8.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Yang, Lizhu,Yun, Wen,Chen, Yilin,et al. Ultrasensitive colorimetric and fluorometric detection of Hg(II) based on the use of gold nanoparticles and a catalytic hairpin assembly[J]. MICROCHIMICA ACTA,2017,184(12):4741-4747.
APA	Yang, Lizhu.,Yun, Wen.,Chen, Yilin.,Wu, Hong.,Liu, Xingyan.,...&Huang, Yu.(2017).Ultrasensitive colorimetric and fluorometric detection of Hg(II) based on the use of gold nanoparticles and a catalytic hairpin assembly.MICROCHIMICA ACTA,184(12),4741-4747.
MLA	Yang, Lizhu,et al."Ultrasensitive colorimetric and fluorometric detection of Hg(II) based on the use of gold nanoparticles and a catalytic hairpin assembly".MICROCHIMICA ACTA 184.12(2017):4741-4747.