Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates
	Li, J.1 ; Zhang, L. L.1,2; Liu, Q. H.1,2; Xu, X. Z.1,2; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.1; Xue, Q. Z.3
刊名	CRYOBIOLOGY
	2009-08-01
卷号	59 期号:1 页码:48-53
关键词	Red Seabream Embryo Extra- And Intra-cellular Ice Formation Cooling Rate
ISSN号	0011-2240
通讯作者	Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
产权排序	Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol;Chinese Acad Sci, Grad Sch;Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev
文献子类	Article
英文摘要	The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on the temperatures of oil globule ice formation (T-OIF), extra-cellular ice formation (T-EIF) and intracellular ice formation (T-IIF) using cryomicroscope. After thawing, the morphological changes of embryos were observed and recorded by the video attachment and monitor under the microscope. During the cooling process, three representative phenomena were observed: oil globule gradually turned bright firstly, then the whole field of view flashed and the embryo blackened. Cooling rate affect the temperature of both extra- and intra-cellular ice formations. T-EIF and T-IIF at high cooling rate were much lower than that at low cooling rate. And the value of T-EIF - T-IIF increased from 0.45 to 11.11 degrees C with the increase of cooling rate from 3 to 130 degrees C/min. Taken together, our results suggested that high cooling rate with proper cryoprotectant would be a good option for red seabream embryo cryopreservation. (C) 2009 Elsevier Inc. All rights reserved.
学科主题	Biology ; Physiology
URL标识	查看原文
WOS关键词	ZEBRAFISH DANIO-RERIO ; PARALICHTHYS-OLIVACEUS EMBRYOS ; SPARUS-AURATA EMBRYOS ; SCOPHTHALMUS-MAXIMUS ; TURBOT EMBRYOS ; HUMAN OOCYTES ; CRYOPRESERVATION ; VITRIFICATION ; SPERM ; CRYOPROTECTANTS
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Physiology
语种	英语
WOS记录号	WOS:000268059000008
资助机构	National Natural Science Foundation of China [30571427]
公开日期	2011-07-05
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/3533]
专题	烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院烟台海岸带研究所知识产出
作者单位	1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Li, J.,Zhang, L. L.,Liu, Q. H.,et al. Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates[J]. CRYOBIOLOGY,2009,59(1):48-53.
APA	Li, J..,Zhang, L. L..,Liu, Q. H..,Xu, X. Z..,Xiao, Z. Z..,...&Xue, Q. Z..(2009).Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates.CRYOBIOLOGY,59(1),48-53.
MLA	Li, J.,et al."Extra- and intra-cellular ice formation of red seabream (Pagrus major) embryos at different cooling rates".CRYOBIOLOGY 59.1(2009):48-53.