Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa

doi:10.1021/acs.analchem.7b02164

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa
	Chen, Guang 1,2,3,4; Fu, Qiang 1,2; Yu, Fabiao 1,2,3; Ren, Rui 4; Liu, Yuxia 1,2; Cao, Ziping 1,2; Li, Guoliang 1,2; Zhao, Xianen 1,2; Chen, Lingxin1,2,3 ; Wang, Hua 1,2
刊名	ANALYTICAL CHEMISTRY
	2017-08-15
卷号	89 期号:16 页码:8509-8516
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b02164
产权排序	第3完成单位
文献子类	Article
英文摘要	The cells control their pH change in a very accurate range. pH plays important roles in cell autophagy and apoptosis. Previous evidence implies that the internal milieu of a tumor is acidified. Although the acidification in cells is investigated, the biological effects from multiple stimulating factors under the complex intracellular environment have not been thoroughly elaborated yet. Currently, there are few pH probes that perform in a wide acidity range, and a probe that is capable of measuring a wide pH range needs to be developed. Herein, we report two new fluorescent probes (BHNBD and CM-BHNBD) for the detection of mitochondrial and intramucosal acidification. The two probes respond to pH via an H+-driven TICT (twist intramolecular charge transfer) mechanism, and they can linearly report pH within a wide pH range: 7.00-2.00 following similar to 148-fold fluorescence increase. The two probes also possess excellent membrane permeability, good photostability, and negligible cytotoxicity. The probes are successfully applied for quantifying the acidification in HeLa cells under the simultaneous stimulation of nutrient deprivation and oxidative stress. Our results demonstrate that the mitochondrial pH is in a dynamic fluctuating state during the acidification process, which suggests a potential cross-talk effect between cell autophagy and apoptosis. We also use the probes for quantifying the intramucosal pH variation in stomach and esophagus via manipulating cellular proton pump. The development of our probes is potentially expected to be used to monitor the intracellular/intramucosal acidification for biomedical research.
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WOS关键词	OXIDATIVE STRESS ; LIVING CELLS ; DESIGN STRATEGIES ; HYDROGEN-PEROXIDE ; MECHANISM ; APOPTOSIS ; AUTOPHAGY ; MITOPHAGY ; CHEMOSELECTIVITY ; HETEROGENEITY
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000407988600047
资助机构	National Natural Science Foundation of China(21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076) ; Shandong Province Key Laboratory of Detection Technology for Tumor Markers(KLDTTM2015-6, KLDTTM2015-9) ; Taishan Scholar Foundation of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/23423]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Yu, Fabiao; Chen, Lingxin; You, Jinmao; Yu, Fabiao(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China); Chen, Lingxin(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China); You, Jinmao(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China)
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Linyi Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Detect Technol Tumor Makers, Linyi 276005, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guang,Fu, Qiang,Yu, Fabiao,et al. Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa[J]. ANALYTICAL CHEMISTRY,2017,89(16):8509-8516.
APA	Chen, Guang.,Fu, Qiang.,Yu, Fabiao.,Ren, Rui.,Liu, Yuxia.,...&You, Jinmao.(2017).Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa.ANALYTICAL CHEMISTRY,89(16),8509-8516.
MLA	Chen, Guang,et al."Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa".ANALYTICAL CHEMISTRY 89.16(2017):8509-8516.