Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 污染过程与控制实验室

	Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos
	Mao, Z. Z.1; Zhang, L. L.1,2; Xu, X. Z.; Liu, Q. H.1,2; Li, J.1 ; Ma, D. Y.1; Xu, S. H.1; Xue, Y. P.; Xue, Q. Z.3
刊名	THERIOGENOLOGY
	2008-10-15
卷号	70 期号:7 页码:1086-1092
关键词	Red Seabream Embryo Cryoprotectant Toxicity Permeable And non-Permeable
通讯作者	Li, J, Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
产权排序	Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D;Chinese Acad Sci, Grad Sch;Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev
文献子类	Article
英文摘要	The objectives were to investigate the effect of cryoprotectants on the hatching rate of red seabream embryos. Heart-beat embryos were immersed in: five permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), 1,2-propylene glycol (PG), and ethylene glycol (EG). in concentrations of 5-30% for 10, 30, or 60 min; and two non-permeable cryoprotectants: polyvinylpyrrolidone (PVP), and sucrose (in concentrations of 5-20% for 10 or 30 min). The embryos were then washed and incubated in filtered seawater until hatching occurred. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. In addition, PG was the least toxic permeable cryoprotectant, followed by DMSO and EG, whereas Gly and MeOH were the most toxic. At a concentration of 15% and 30 min exposure, the hatching rate of the embryos immersed in PG was 93.3 +/- 7.0% (mean +/- S.D.), however. in DMSO. EG, Gly. and MeOH, it was 82.7 +/- 10.4, 22.0 +/- 5.7, 0.0 +/- 0.0, and 0.0 +/- 0.0%, respectively. Hatching rate of embryos treated with PVP decreased (P < 0.05) with the increase of concentration and exposure time, whereas for embryos treated with sucrose, there was no significant decrease in comparison with the control at the concentrations used. (C) 2008 Elsevier Inc. All rights reserved.
学科主题	Reproductive Biology ; Veterinary Sciences
WOS关键词	PARALICHTHYS-OLIVACEUS EMBRYOS ; ZEBRAFISH DANIO-RERIO ; SPARUS-AURATA EMBRYOS ; CHILLING SENSITIVITY ; SCOPHTHALMUS-MAXIMUS ; BRACHYDANIO-RERIO ; CRASSOSTREA-GIGAS ; CRYOPRESERVATION ; TOXICITY ; VITRIFICATION
WOS研究方向	Reproductive Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000259750000009
资助机构	National Natural Science Foundation of China [30571427]
公开日期	2010-09-29
内容类型	期刊论文
源URL	[http://lib.yic.ac.cn/handle/133337/1221]
专题	烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院烟台海岸带研究所知识产出
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Mao, Z. Z.,Zhang, L. L.,Xu, X. Z.,et al. Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos[J]. THERIOGENOLOGY,2008,70(7):1086-1092.
APA	Mao, Z. Z..,Zhang, L. L..,Xu, X. Z..,Liu, Q. H..,Li, J..,...&Xue, Q. Z..(2008).Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos.THERIOGENOLOGY,70(7),1086-1092.
MLA	Mao, Z. Z.,et al."Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos".THERIOGENOLOGY 70.7(2008):1086-1092.