Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome

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	Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome
	Liu, Ping 1,2; Wang, Yuan-yuan 1; Qi, Xin 1; Gu, QianQun 1; Geng, Meiyu 3; Li, Jing 1
刊名	PLOS ONE
	2013-06-14
卷号	8 期号:6 页码:e65381
关键词	Cycloprodigiosin Hydrochloride h+/cl-symporter Hl-60 Cells h+-atpase In-vitro Prodigiosin Lines Differentiation Proliferation Inhibition
ISSN号	1932-6203
通讯作者	Geng, MY (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China. mygeng@mail.shcnc.ac.cn ; lijing_ouc@ouc.edu.cn
产权排序	[Liu, Ping; Wang, Yuan-yuan; Qi, Xin; Gu, QianQun; Li, Jing] Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China; [Liu, Ping] Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Geng, Meiyu] Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China
文献子类	Article
英文摘要	Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs.; Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs.
学科主题	Multidisciplinary Sciences
URL标识	查看原文
WOS关键词	CYCLOPRODIGIOSIN HYDROCHLORIDE ; H+/CL-SYMPORTER ; HL-60 CELLS ; H+-ATPASE ; IN-VITRO ; PRODIGIOSIN ; LINES ; DIFFERENTIATION ; PROLIFERATION ; INHIBITION
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000320363300018
资助机构	National High-tech R&D Program of China [2011AA09070104]; Program for Changjiang Scholars and Innovative Research Team in University [IRT0944]
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6475]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China 2.Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ping,Wang, Yuan-yuan,Qi, Xin,et al. Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome[J]. PLOS ONE,2013,8(6):e65381.
APA	Liu, Ping,Wang, Yuan-yuan,Qi, Xin,Gu, QianQun,Geng, Meiyu,&Li, Jing.(2013).Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome.PLOS ONE,8(6),e65381.
MLA	Liu, Ping,et al."Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome".PLOS ONE 8.6(2013):e65381.