Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China
	Wang, Jingjing 1,3; Zhang, Huiwen 1; Zhang, Xiaoli2 ; Qin, Shenghong 1,3; Tan, Huanbo 1,3; Li, Xinyu 1
刊名	ANNALS OF MICROBIOLOGY
	2013-03-01
卷号	63 期号:1 页码:335-341
关键词	Soil Pseudomonas Antifungal Activity Antifungal Metabolites Chlorimuron-ethyl Chronic Effect Ardra Analysis
ISSN号	1590-4261
通讯作者	Li, XY (reprint author), Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China. xyli@iae.ac.cn
产权排序	[Wang, Jingjing; Zhang, Huiwen; Qin, Shenghong; Tan, Huanbo; Li, Xinyu] Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China; [Zhang, Xiaoli] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Jingjing; Qin, Shenghong; Tan, Huanbo] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	The herbicide chlorimuron-ethyl has been applied widely for weed control in farmland, especially in soybean fields in China over the past decade, but the chronic effects of this herbicide on soil microorganisms, particularly Pseudomonas spp., is not well understood. Taking a continuously cropped soybean field in the town of Fuyuan-a soybean production base of Heilongjiang Province in Northeast China-as a case study, soil samples were collected from plots having received 0-, 5-, and 10-year applications of chlorimuron-ethyl (30 g active component of chlorimuron-ethyl/ha/year) to study the abundance and diversity of Pseudomonas spp. Meanwhile, an in vitro assay was used to examine the antifungal activities of isolated Pseudomonas spp. against soil-borne pathogens (Fusarium graminearum, Fusarium oxysporum, and Rhizoctonia solani) causing soybean root rot disease. The production of siderophore, hydrogen cyanide (HCN), and lytic enzymes (cellulase, pectinase, and chitinase) by Pseudomonas spp. was also investigated. With 5- and 10- year chlorimuron-ethyl application, the numbers of soil Pseudomonas spp. decreased from 121 x 10(2) CFU/g dry soil in the control to 40 x 10(2) CFU/g dry soil and 13 x 10(2) CFU/g dry soil, and the Shannon index values decreased from 6.23 to 3.71 and 1.73, respectively. The numbers of antifungal Pseudomonas spp. also decreased, and the proportions of Pseudomonas spp. with antifungal activities against the different test pathogens altered. All the antifungal Pseudomonas spp. could produce siderophore and HCN but not lytic enzymes. The results suggest that long-term application of chlorimuron-ethyl in continuously cropped soybean field had negative effects on the abundance and diversity of soil Pseudomonas spp., including species with different antifungal activities against pathogens. Siderophore and HCN rather than lytic enzymes formed the antifungal metabolites of Pseudomonas spp., and the number of antifungal Pseudomonas that can produce siderophore and HCN decreased markedly under application of chlorimuron-ethyl, especially after 10-year application.; The herbicide chlorimuron-ethyl has been applied widely for weed control in farmland, especially in soybean fields in China over the past decade, but the chronic effects of this herbicide on soil microorganisms, particularly Pseudomonas spp., is not well understood. Taking a continuously cropped soybean field in the town of Fuyuan-a soybean production base of Heilongjiang Province in Northeast China-as a case study, soil samples were collected from plots having received 0-, 5-, and 10-year applications of chlorimuron-ethyl (30 g active component of chlorimuron-ethyl/ha/year) to study the abundance and diversity of Pseudomonas spp. Meanwhile, an in vitro assay was used to examine the antifungal activities of isolated Pseudomonas spp. against soil-borne pathogens (Fusarium graminearum, Fusarium oxysporum, and Rhizoctonia solani) causing soybean root rot disease. The production of siderophore, hydrogen cyanide (HCN), and lytic enzymes (cellulase, pectinase, and chitinase) by Pseudomonas spp. was also investigated. With 5- and 10- year chlorimuron-ethyl application, the numbers of soil Pseudomonas spp. decreased from 121 x 10(2) CFU/g dry soil in the control to 40 x 10(2) CFU/g dry soil and 13 x 10(2) CFU/g dry soil, and the Shannon index values decreased from 6.23 to 3.71 and 1.73, respectively. The numbers of antifungal Pseudomonas spp. also decreased, and the proportions of Pseudomonas spp. with antifungal activities against the different test pathogens altered. All the antifungal Pseudomonas spp. could produce siderophore and HCN but not lytic enzymes. The results suggest that long-term application of chlorimuron-ethyl in continuously cropped soybean field had negative effects on the abundance and diversity of soil Pseudomonas spp., including species with different antifungal activities against pathogens. Siderophore and HCN rather than lytic enzymes formed the antifungal metabolites of Pseudomonas spp., and the number of antifungal Pseudomonas that can produce siderophore and HCN decreased markedly under application of chlorimuron-ethyl, especially after 10-year application.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
URL标识	查看原文
WOS关键词	FLUORESCENT PSEUDOMONADS ; ANTIBIOTIC PRODUCTION ; MICROBIAL DIVERSITY ; RHIZOSPHERE ; PLANT ; PCR
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000314692400036
资助机构	National High Technology Research and Development Program (863 Program) [2012AA101403]; National Science Foundation [41071202]
公开日期	2013-08-14
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6433]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jingjing,Zhang, Huiwen,Zhang, Xiaoli,et al. Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China[J]. ANNALS OF MICROBIOLOGY,2013,63(1):335-341.
APA	Wang, Jingjing,Zhang, Huiwen,Zhang, Xiaoli,Qin, Shenghong,Tan, Huanbo,&Li, Xinyu.(2013).Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China.ANNALS OF MICROBIOLOGY,63(1),335-341.
MLA	Wang, Jingjing,et al."Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China".ANNALS OF MICROBIOLOGY 63.1(2013):335-341.