Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus

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	Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus
	Wei, Xiumei 2; Liu, Xiangquan 2; Yang, Jianmin 2; Fang, Jinghui 2; Qiao, Hongjin 2; Zhang, Ying 2; Yang, Jialong 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012
卷号	32 期号:1 页码:132-140
关键词	C-type Lectin Litopenaeus Vannamei Innate Immunity Pathogen Recognition Quantitative Real-time Pcr
ISSN号	1050-4648
通讯作者	Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. ladderup@126.com ; jlyang@yic.ac.cn
产权排序	[Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Liu, Xiangquan; Yang, Jianmin; Fang, Jinghui; Qiao, Hongjin; Zhang, Ying] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
文献子类	Article
英文摘要	C-type lectins play crucial roles in innate immunity to recognize and eliminate pathogens efficiently. In the present study, two C-type lectins from shrimp Litopenaeus vannamei (designated as LvLectin-1 and LvLectin-2) were identified, and their expression patterns, both in tissues and toward pathogen stimulation, were then characterized. The full-length cDNA of LvLectin-1 and LvLectin-2 was 567 and 625 bp, containing an open reading frame (ORF) of 471 and 489 bp, respectively, and deduced amino acid sequences showed high similarity to other members of C-type lectin superfamily. Both two C-type lectins encoded a single carbohydrate-recognition domain (CRD). The motif of Ca2+ binding site 2 in CRD, which determined carbohydrate-binding specificity, was QPN (GIn(122)-Pro(123)-Asn(124)) in LvLectin-1, but QPD (Gln(128)-Pro(129)-Asp(130)) in LvLectin-2. Two C-type lectins exhibited similar tissue expression pattern, for their mRNA were both constitutively expressed in all tested tissues, including hepatopancreas, muscle, gill, hemocytes, gonad and heart, furthermore they were both mostly expressed in hepatopancreas, though the expression level of LvLectin-2 was much higher than LvLectin-1. The expression level of two C-type lectins mRNA in hemocytes varied greatly after the challenge of Listonella anguillarum or WSSV. After L. anguillarum challenge, the expression of both C-type lectins were significantly (P < 0.01) up-regulated compared with blank group, and LvLectin-1 exhibited higher level than LvLectin-2; while after the stimulation of WSSV, the expression of LvLectin-2 was significantly up-regulated at 6 h (P < 0.01) and 12 h (P < 0.05), but the expression level of LvLectin-1 down-regulated significantly (P < 0.01) to 0.4-fold at 6 and 12 h post-stimulation. The results indicated that the two C-type lectins might be involved in immune response toward pathogen infection, and they might perform different recognition specificity toward bacteria or virus.; C-type lectins play crucial roles in innate immunity to recognize and eliminate pathogens efficiently. In the present study, two C-type lectins from shrimp Litopenaeus vannamei (designated as LvLectin-1 and LvLectin-2) were identified, and their expression patterns, both in tissues and toward pathogen stimulation, were then characterized. The full-length cDNA of LvLectin-1 and LvLectin-2 was 567 and 625 bp, containing an open reading frame (ORF) of 471 and 489 bp, respectively, and deduced amino acid sequences showed high similarity to other members of C-type lectin superfamily. Both two C-type lectins encoded a single carbohydrate-recognition domain (CRD). The motif of Ca2+ binding site 2 in CRD, which determined carbohydrate-binding specificity, was QPN (GIn(122)-Pro(123)-Asn(124)) in LvLectin-1, but QPD (Gln(128)-Pro(129)-Asp(130)) in LvLectin-2. Two C-type lectins exhibited similar tissue expression pattern, for their mRNA were both constitutively expressed in all tested tissues, including hepatopancreas, muscle, gill, hemocytes, gonad and heart, furthermore they were both mostly expressed in hepatopancreas, though the expression level of LvLectin-2 was much higher than LvLectin-1. The expression level of two C-type lectins mRNA in hemocytes varied greatly after the challenge of Listonella anguillarum or WSSV. After L. anguillarum challenge, the expression of both C-type lectins were significantly (P < 0.01) up-regulated compared with blank group, and LvLectin-1 exhibited higher level than LvLectin-2; while after the stimulation of WSSV, the expression of LvLectin-2 was significantly up-regulated at 6 h (P < 0.01) and 12 h (P < 0.05), but the expression level of LvLectin-1 down-regulated significantly (P < 0.01) to 0.4-fold at 6 and 12 h post-stimulation. The results indicated that the two C-type lectins might be involved in immune response toward pathogen infection, and they might perform different recognition specificity toward bacteria or virus. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS关键词	CARBOHYDRATE-RECOGNITION DOMAINS ; SCALLOP CHLAMYS-FARRERI ; MANDUCA-SEXTA ; ANIMAL LECTINS ; FENNEROPENAEUS-CHINENSIS ; MOLECULAR-CLONING ; PENAEUS-MONODON ; BINDING PROTEIN ; CDNA CLONING ; WHITE SHRIMP
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000299979100016
资助机构	Shandong Provincial Natural Science Foundation, China[ZR2011CQ017]
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5586]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Wei, Xiumei,Liu, Xiangquan,Yang, Jianmin,et al. Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(1):132-140.
APA	Wei, Xiumei.,Liu, Xiangquan.,Yang, Jianmin.,Fang, Jinghui.,Qiao, Hongjin.,...&Yang, Jialong.(2012).Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus.FISH & SHELLFISH IMMUNOLOGY,32(1),132-140.
MLA	Wei, Xiumei,et al."Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus".FISH & SHELLFISH IMMUNOLOGY 32.1(2012):132-140.