Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial
	He, Weiyu 1; Feng, Feifei 1; Huang, Yong 4; Guo, Huanhuan 1; Zhang, Songyan 2; Li, Zheng 2; Liu, Jingze 1; Wang, Yipeng3 ; Yu, Haining 1,2
刊名	BIOCHIMIE
	2012-03-01
卷号	94 期号:3 页码:649-655
关键词	Amphibian Diversity Anti-microbial Peptide (Amp) Antioxidant Odorrana Tiannanensis
ISSN号	0300-9084
通讯作者	Yu, HN (reprint author), Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China. wyp010@163.com ; joannyu@live.cn
产权排序	[He, Weiyu; Feng, Feifei; Guo, Huanhuan; Liu, Jingze; Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Zhang, Songyan; Li, Zheng; Yu, Haining] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Wang, Yipeng] Chinese Acad Sci, Biol Resources Lab, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Huang, Yong] Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China
文献子类	Article
英文摘要	Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear.; Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear. (C) 2011 Elsevier Masson SAS. All rights reserved.
学科主题	Biochemistry & Molecular Biology
URL标识	查看原文
WOS关键词	AMPHIBIAN SKIN ; PEPTIDOMICS ; INDUCTION ; ROLES
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000301332200008
资助机构	Chinese National Natural Science Foundation[30900240, 41076098]
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5562]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China 2.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Biol Resources Lab, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 4.Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China
推荐引用方式 GB/T 7714	He, Weiyu,Feng, Feifei,Huang, Yong,et al. Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial[J]. BIOCHIMIE,2012,94(3):649-655.
APA	He, Weiyu.,Feng, Feifei.,Huang, Yong.,Guo, Huanhuan.,Zhang, Songyan.,...&Yu, Haining.(2012).Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial.BIOCHIMIE,94(3),649-655.
MLA	He, Weiyu,et al."Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial".BIOCHIMIE 94.3(2012):649-655.