Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7
	Lu, Yandu 1,2,3; Chi, Xiaoyuan 4; Yang, Qingli 4; Li, Zhaoxin 5; Liu, Shaofang 2,3; Gan, Qinhua 6; Qin, Song1
刊名	EXTREMOPHILES
	2009-11-01
卷号	13 期号:6 页码:875-884
关键词	Cold Stress High Salinity Stress Antarctic Delta(12) Fatty Acid Desaturases
ISSN号	1431-0651
通讯作者	Qin, S, Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
产权排序	Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev;Chinese Acad Sci, Inst Oceanol;Chinese Acad Sci, Grad Sch;Shandong Peanut Res Inst;Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst;Shandong Entry Exit Inspect & Quarantine Bur
文献子类	Article
英文摘要	The psychrotrophic Antarctic alga, Chlorella vulgaris NJ-7, grows under an extreme environment of low temperature and high salinity. In an effort to better understand the correlation between fatty acid metabolism and acclimation to Antarctic environment, we analyzed its fatty acid compositions. An extremely high amount of Delta(12) unsaturated fatty acids was identified which prompted us to speculate about the involvement of Delta(12) fatty acid desaturase in the process of acclimation. A full-length cDNA sequence, designated CvFAD2, was isolated from C. vulgaris NJ-7 via reverse transcription polymerase chain reaction (RT-PCR) and RACE methods. Sequence alignment and phylogenetic analysis showed that the gene was homologous to known microsomal Delta(12)-FADs with the conserved histidine motifs. Heterologous expression in yeast was used to confirm the regioselectivity and the function of CvFAD2. Linoleic acid (18:2), normally not present in wild-type yeast cells, was detected in transformants of CvFAD2. The induction of CvFAD2 at an mRNA level under cold stress and high salinity is detected by real-time PCR. The results showed that both temperature and salinity motivated the upregulation of CvFAD2 expression. The accumulation of CvFAD2 increased 2.2-fold at 15A degrees C and 3.9-fold at 4A degrees C compared to the alga at 25A degrees C. Meanwhile a 1.7- and 8.5-fold increase at 3 and 6% NaCl was detected. These data suggest that CvFAD2 is the enzyme responsible for the Delta(12) fatty acids desaturation involved in the adaption to cold and high salinity for Antarctic C. vugaris NJ-7.
学科主题	Biochemistry & Molecular Biology ; Microbiology
URL标识	查看原文
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; FUNCTIONAL-CHARACTERIZATION ; MEMBRANE-FLUIDITY ; PAVLOVA-SALINA ; SPLICE SITES ; YEAST ; IDENTIFICATION ; ENZYME ; CHLOROPHYCEAE ; BIOSYNTHESIS
WOS研究方向	Biochemistry & Molecular Biology ; Microbiology
语种	英语
WOS记录号	WOS:000271199500003
资助机构	National High-Tech Research and Development Plan of China [2006AA10A114]; National Key Basic Research and Development Project of China [2007CB116212]; Key Innovative Project of Chinese Academy of Science [KSCX2-YW-G-002, KZCX2-YW-225]; National Natural Sc
公开日期	2011-07-05
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/3476]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China 5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 6.Shandong Entry Exit Inspect & Quarantine Bur, Qingdao 266001, Peoples R China
推荐引用方式 GB/T 7714	Lu, Yandu,Chi, Xiaoyuan,Yang, Qingli,et al. Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7[J]. EXTREMOPHILES,2009,13(6):875-884.
APA	Lu, Yandu.,Chi, Xiaoyuan.,Yang, Qingli.,Li, Zhaoxin.,Liu, Shaofang.,...&Qin, Song.(2009).Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7.EXTREMOPHILES,13(6),875-884.
MLA	Lu, Yandu,et al."Molecular cloning and stress-dependent expression of a gene encoding Delta(12)-fatty acid desaturase in the Antarctic microalga Chlorella vulgaris NJ-7".EXTREMOPHILES 13.6(2009):875-884.