MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides
	Ni, SW; Yu, YP; Wei, JG; Zhou, LL; Wei, SN; Yan, Y; Huang, XH; Huang, YH; Qin, QW
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018
卷号	72 页码:9-13
通讯作者	huangyh@scsio.ac.cn ; qinqw@scsio.ac.cn
英文摘要	MicroRNA-146a (miR-146a) has been demonstrated to function as a negative regulator of cellular immune responses against pathogens in mammals, however, little information focused on its functions in lower vertebrates. In this study, we investigated the regulatory roles of orange spotted grouper, Epinephelus coioides miR-146a during red spotted grouper nervous necrosis virus (RGNNV) infection. During RGNNV infection in grouper spleen (GS) cells, the endogenous expression level of miR-146a and tumor necrosis factor receptor-associated factor 6 (TRAF6) significantly increased along with the infection time. Overexpression of miR-146a significantly facilitated viral infection, evidenced by the increased transcription of viral CP and RdRp genes, while miR-146a knockdown by specific inhibitors decreased RGNNV replication. Using pMIR-REPORT Luciferase system, we found that the 3' untranslated region (UTR) of grouper TRAF6 could be specifically targeted by miR-146a. Further studies showed that its downstream target gene pro-inflammatory cytokines, including TNF-alpha, IL-8 and IL-beta, were all significantly decreased in miR-146a mimic transfected cells, but increased in miR-146a inhibitors transfected cells during RGNNV infection. Thus, our results suggested and verified that holding the level of miR-146a exerted crucial roles in RGNNV infection through TRAF6-mediated inflammatory response.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
关键词[WOS]	miR-146a ; RGNNV ; Grouper ; TRAF6 ; Viral infection
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17030]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Ni, SW,Yu, YP,Wei, JG,et al. MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:9-13.
APA	Ni, SW.,Yu, YP.,Wei, JG.,Zhou, LL.,Wei, SN.,...&Qin, QW.(2018).MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,72,9-13.
MLA	Ni, SW,et al."MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 72(2018):9-13.