Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)
	Liang, YS; Ding, X; Yu, X; Wang, Y; Zhou, Y; He, JA; Shi, Y; Zhang, Y; Lin, HR; Lu, DQ
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018
卷号	74 页码:309-317
通讯作者	ludanqi@mail.sysu.edu.cn
英文摘要	Toll-like receptors (TLRs) are one of the most important innate immune receptors, which recognize various pathogen-associated molecular patterns and activate the downstream immune response. Mouse TLR13 has been found to recognize a highly conserved sequence from bacterial or viral RNA and activate the myeloid differentiation primary response gene 88-dependent signaling response. The function of teleost tlr13 is still not fully understood, especially its relationship with bacterial RNA. In our study, we identified and characterized a tlr13 from Epinephelus coioides (orange-spotted grouper). The full-length cDNA of Eco. tlr13 contained a 2844 bp open reading frame, encoding 947 amino acids. The polypeptide was constitutive of a signal peptide, 13 leucine-rich repeats domains, a C-terminal leucine-rich repeats, a transmembrane domain and a conserved Toll/interleukin (IL)-1 receptor domain, indicating that Eco. Tlr13 exhibited a typical TLR structure. Multiple alignments showed that the Toll/IL-1 receptor domain of Eco. Tlr13 was identical with other homologues, and the phylogenetic tree suggested that Eco. Tlr13 was clustered with other TLR13s and had the closest relationship with predicted Lates calcarifer (sea bass) Tlr13. Subcellular localization analysis revealed that Eco. Tlr13 colocalized with the endoplasmic reticulum and early endosome. Moreover, Eco. tlr13 was broadly observed in all tested tissues with the relatively high expressions in the brain and immune-related tissues. After challenged with 19-mer Staphylococcus aureus 23S ribosomal RNA-derived oligoribonucleotide (ORN Sa19), the expression of Eco. tlr13 was significantly up-regulated in grouper spleen cells. Also, the luciferase assay further revealed that with the overexpression of Eco. Tlr13 in human embryonic kidney 293T cells, ORN Sa19 activated the promoter activity of interferon-beta in a dose-dependent pattern. These results indicate that Eco. tlr13 may involve in the recognition of bacterial RNA.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
关键词[WOS]	Epinephelus coioides ; tlr13 ; Subcellular distribution ; ORN Sa19
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/16991]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Liang, YS,Ding, X,Yu, X,et al. Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:309-317.
APA	Liang, YS.,Ding, X.,Yu, X.,Wang, Y.,Zhou, Y.,...&Lu, DQ.(2018).Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,74,309-317.
MLA	Liang, YS,et al."Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 74(2018):309-317.