Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening

doi:10.1016/j.etap.2017.07.005

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	Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening
	Zhang, Sen 1,2,3; Chen, Huan 3; Wang, An 1; Liu, Yong 1; Hou, Hongwei 3; Hu, Qingyuan 3
刊名	ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
	2017-10-01
卷号	55 页码:30-36
关键词	Gamma H2ax High Content Screening Aldehyde Benzene Dose/time-response
DOI	10.1016/j.etap.2017.07.005
文献子类	Article
英文摘要	To evaluate the genotoxic effects of formaldehyde, acetaldehyde, acrolein and benzene on A549 cells, the in vitro gamma H2AX assay was used in combination with high content screening (HCS) technology. All aldehydes showed a significant genotoxicity in a dose/time-dependent effect on the induction of gamma H2AX. Benzene failed to show a significant genotoxicity based on the gamma H2AX assay. However, hydroquinone (one of metabolites of benzene) showed a significant genotoxicity in vitro. Based on the dose-response of gamma H2AX and Hill model, the ability to induce DNA double-strand break can be evaluated as acrolein > formaldehyde > acetaldehyde > benzene. The slow DNA damage/repair mechanism may be more important than the fast one for aldehydes based on time course of gamma H2AX and two-component model. Overall, all toxicants were genotoxic in a dose- or time-dependent manner based on the in vitro gamma H2AX HCS assay, and acrolein had a strong potential to induce DNA damage followed by formaldehyde, acetaldehyde and benzene in sequence.
WOS关键词	INDUCED DNA-DAMAGE ; DOSE-RESPONSE
WOS研究方向	Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
语种	英语
WOS记录号	WOS:000413383100005
资助机构	National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002) ; National Natural Science Foundation of China(21347002)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/33831]
专题	合肥物质科学研究院_应用技术研究所
作者单位	1.Chinese Acad Sci, Hefei Inst phys Sci, Inst Appl Technol, Hefei 230088, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.China Natl Tobacco Qual Supervis & Test Ctr, Zhengzhou 450001, Henan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Sen,Chen, Huan,Wang, An,et al. Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2017,55:30-36.
APA	Zhang, Sen,Chen, Huan,Wang, An,Liu, Yong,Hou, Hongwei,&Hu, Qingyuan.(2017).Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,55,30-36.
MLA	Zhang, Sen,et al."Assessment of genotoxicity of four volatile pollutants from cigarette smoke based on the in vitro gamma H2AX assay using high content screening".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 55(2017):30-36.