Conditional control of suicide gene expression in tumor cells with theophylline-responsive ribozyme

doi:10.1038/gt.2016.78

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Conditional control of suicide gene expression in tumor cells with theophylline-responsive ribozyme
	Zhang, Y.4,5,6; Wang, J.6; Cheng, H.6; Sun, Y.6 ; Liu, M.6; Wu, Z.4; Pei, R.6
刊名	GENE THERAPY
	2017-02-01
卷号	24 期号:2 页码:84-91
DOI	10.1038/gt.2016.78
文献子类	Article
英文摘要	Numerous synthetic RNA-based controls for integrating sensing switches with function devices have been demonstrated in a variety of organisms for gene regulation. Although potential advantages of RNA-based genetic control strategies have been shown in clinical applications, successfully extending these engineered systems into medical applications has seldom been reported. Here, a synthetic RNA-based ribozyme system and its application in advancing rationally designed cellular therapy were described. The theophylline-responsive, ribozyme-based device provided a powerful platform for suicide gene expression regulation in tumor cells. Moreover, we demonstrate the ability of our synthetic controller to modulate effectively the viability of the cells in response to drug input. Our RNA-based regulatory system could dose-dependently fine-tune transgene expression in mammalian cells and address urgent limitations in existing genetic control strategies for gene-and cell-based therapies in the future.
WOS关键词	VERSUS-LEUKEMIA REACTIONS ; MARROW TRANSPLANTATION ; DONOR LYMPHOCYTES ; T-CELLS ; RNA ; IMMUNOTHERAPY ; RIBOSWITCHES ; THERAPY
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
语种	英语
WOS记录号	WOS:000394682800005
资助机构	National Natural Science Foundation of China(31300096 ; National Natural Science Foundation of China(31300096 ; National Natural Science Foundation of China(31300096 ; National Natural Science Foundation of China(31300096 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; CAS/SAFEA International Innovation Teams program ; CAS/SAFEA International Innovation Teams program ; CAS/SAFEA International Innovation Teams program ; CAS/SAFEA International Innovation Teams program ; 21275156) ; 21275156) ; 21275156) ; 21275156) ; BK20130357) ; BK20130357) ; BK20130357) ; BK20130357) ; National Natural Science Foundation of China(31300096 ; National Natural Science Foundation of China(31300096 ; National Natural Science Foundation of China(31300096 ; National Natural Science Foundation of China(31300096 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; Natural Science Foundation of Jiangsu Province(BK20130351 ; CAS/SAFEA International Innovation Teams program ; CAS/SAFEA International Innovation Teams program ; CAS/SAFEA International Innovation Teams program ; CAS/SAFEA International Innovation Teams program ; 21275156) ; 21275156) ; 21275156) ; 21275156) ; BK20130357) ; BK20130357) ; BK20130357) ; BK20130357)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/32936]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Suzhou Inst Nano Tech & Nano Bion, Suzhou 215123, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 5.Univ Sci & Technol China, Hefei 230026, Peoples R China 6.Chinese Acad Sci, Suzhou Inst Nano Tech & Nano Bion, Suzhou 215123, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Y.,Wang, J.,Cheng, H.,et al. Conditional control of suicide gene expression in tumor cells with theophylline-responsive ribozyme[J]. GENE THERAPY,2017,24(2):84-91.
APA	Zhang, Y..,Wang, J..,Cheng, H..,Sun, Y..,Liu, M..,...&Pei, R..(2017).Conditional control of suicide gene expression in tumor cells with theophylline-responsive ribozyme.GENE THERAPY,24(2),84-91.
MLA	Zhang, Y.,et al."Conditional control of suicide gene expression in tumor cells with theophylline-responsive ribozyme".GENE THERAPY 24.2(2017):84-91.