Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy

doi:10.1177/0003702816645605

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy
	Liu, Jinghua 1,2; Huang, Qing 1,2,3
刊名	APPLIED SPECTROSCOPY
	2016-10-01
卷号	70 期号:10 页码:1639-1648
关键词	Astaxanthin Fourier Transform Infrared Ft-ir Microspectroscopy Haematococcus Pluvialis Raman Microspectroscopy
DOI	10.1177/0003702816645605
文献子类	Article
英文摘要	Haematococcus pluvialis has promising applications owing to its ability to accumulate astaxanthin under stress conditions. In order to acquire higher astaxanthin productivity from H. pluvialis, it is critical not only to develop efficient mutagenesis techniques, but also to establish rapid and effective screening methods which are highly demanded in current research and application practice. In this work, we therefore attempted to develop a new approach to screening the astaxanthin-hyperproducing strains based on spectroscopic tools. Using Fourier transform infrared (FT-IR) and Raman microspectroscopy, we have achieved rapid and quantitative analysis of the algal cells in terms of astaxanthin, beta-carotene, proteins, lipids, and carbohydrates. In particular, we have found that the ratio of the IR absorption band at 1740 cm(-1) to the band at 1156 cm(-1) can be utilized for identifying astaxanthin-hyperproducing strains. This work may therefore open a new avenue for developing high-throughput screening methods necessary for the microbial mutant breeding industry.
WOS关键词	MULTIVARIATE CURVE RESOLUTION ; CAROTENOGENIC GENES ; YEAST-CELL ; SPECTROSCOPY ; MICROALGAE ; MICROSCOPY ; SCATTERING ; MUTANTS ; STRESS ; ACCUMULATION
WOS研究方向	Instruments & Instrumentation ; Spectroscopy
语种	英语
WOS记录号	WOS:000386026300003
资助机构	National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; National Natural Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/30143]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China 3.Univ Sci & Technol China, Sch Nucl Sci & Technol, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jinghua,Huang, Qing. Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy[J]. APPLIED SPECTROSCOPY,2016,70(10):1639-1648.
APA	Liu, Jinghua,&Huang, Qing.(2016).Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy.APPLIED SPECTROSCOPY,70(10),1639-1648.
MLA	Liu, Jinghua,et al."Screening of Astaxanthin-Hyperproducing Haematococcus pluvialis Using Fourier Transform Infrared (FT-IR) and Raman Microspectroscopy".APPLIED SPECTROSCOPY 70.10(2016):1639-1648.