Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy

doi:10.1021/acs.analchem.6b00400

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	Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy
	Zhang, Fengqiu 1,2,3; Huang, Qing 1,2; Yan, Jingwen 1; Chen, Zhu 1
刊名	ANALYTICAL CHEMISTRY
	2016-04-19
卷号	88 期号:8 页码:4179-4182
DOI	10.1021/acs.analchem.6b00400
文献子类	Article
英文摘要	A nucleosome is made up of DNA and histones, and acetylation of histones perturbs the interaction of DNA and histones and thus affects the chromatin conformation and function. However, whether or how acetylation induces DNA conformation changes is still elusive. In this work, we applied FT-IR spectroscopy to monitor the DNA signals in cells as the histone acetylation was regulated by trichostatin A (TSA), a reversible inhibitor to histone deacetylases (HDACs). Our results unambiguously demonstrate the significant transformation of B-DNA to Z-DNA upon histone acetylation in the TSA treated HeLa cells: This is the first report providing the explicit experimental evidence for such a B-Z transformation of DNA in the epigenetic states of cells.
WOS关键词	LINKING NUMBER CHANGE ; TRICHOSTATIN-A ; INFRARED MICROSPECTROSCOPY ; DEACETYLASE INHIBITORS ; CHROMATIN STRUCTURE ; NUCLEIC-ACIDS ; DIFFERENTIATION ; CANCER ; THERAPY ; ACETYLTRANSFERASES
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000374706000003
资助机构	Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; 2013CB934304) ; 2013CB934304) ; 2013CB934304) ; 2013CB934304) ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; Major National Scientific Research Project(2014CB932002 ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; National Nature Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107) ; 2013CB934304) ; 2013CB934304) ; 2013CB934304) ; 2013CB934304)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22396]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Key Lab Ion Beam Bioengn, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei, Peoples R China 2.Univ Sci & Technol China, Sch Nucl Sci & Technol, Hefei 230026, Peoples R China 3.Zhengzhou Univ, Sch Phys Engn, Henan Key Lab Ion Beam Bioengn, Zhengzhou 450052, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fengqiu,Huang, Qing,Yan, Jingwen,et al. Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy[J]. ANALYTICAL CHEMISTRY,2016,88(8):4179-4182.
APA	Zhang, Fengqiu,Huang, Qing,Yan, Jingwen,&Chen, Zhu.(2016).Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy.ANALYTICAL CHEMISTRY,88(8),4179-4182.
MLA	Zhang, Fengqiu,et al."Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy".ANALYTICAL CHEMISTRY 88.8(2016):4179-4182.