The role of protein kinase C alpha translocation in radiation-induced bystander effect

doi:10.1038/srep25817

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	The role of protein kinase C alpha translocation in radiation-induced bystander effect
	Fang, Zihui 1; Xu, An 2; Wu, Lijun 2; Hei, Tom K.3; Hong, Mei 1
刊名	SCIENTIFIC REPORTS
	2016-05-11
卷号	6 期号:无页码:1-9
DOI	10.1038/srep25817
文献子类	Article
英文摘要	Ionizing radiation is a well known human carcinogen. Evidence accumulated over the past decade suggested that extranuclear/extracellular targets and events may also play a critical role in modulating biological responses to ionizing radiation. However, the underlying mechanism(s) of radiation-induced bystander effect is still unclear. In the current study, A(L) cells were irradiated with alpha particles and responses of bystander cells were investigated. We found out that in bystander AL cells, protein kinase C alpha (PKCZ alpha) translocated from cytosol to membrane fraction. Pre-treatment of cells with PKC translocation inhibitor chelerythrine chloride suppressed the induced extracellular signal-regulated kinases (ERK) activity and the increased cyclooxygenase 2 (COX-2) expression as well as the mutagenic effect in bystander cells. Furthermore, tumor necrosis factor alpha (TNF alpha) was elevated in directly irradiated but not bystander cells; while TNFa receptor 1 (TNFR1) increased in the membrane fraction of bystander cells. Further analysis revealed that PKC activation caused accelerated internalization and recycling of TNFR1. Our data suggested that PKC alpha translocation may occur as an early event in radiation-induced bystander responses and mediate TNF alpha-induced signaling pathways that lead to the activation of ERK and up-regulation of COX-2.
WOS关键词	MOUSE EPIDERMAL-CELLS ; GENOMIC INSTABILITY ; IONIZING-RADIATION ; HUMAN FIBROBLASTS ; MAMMALIAN-CELLS ; PATHWAY ; SENSITIVITY ; ACTIVATION ; EXPRESSION ; PARTICLES
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000375615100001
资助机构	National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; 81373473 ; 81373473 ; 81373473 ; 81373473 ; U1332124) ; U1332124) ; U1332124) ; U1332124) ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; National Natural Science Foundation of China(11335011 ; 81373473 ; 81373473 ; 81373473 ; 81373473 ; U1332124) ; U1332124) ; U1332124) ; U1332124)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22381]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 3.Columbia Univ Coll Phys & Surg, Ctr Radiol Res, 630 W 168th St, New York, NY 10032 USA
推荐引用方式 GB/T 7714	Fang, Zihui,Xu, An,Wu, Lijun,et al. The role of protein kinase C alpha translocation in radiation-induced bystander effect[J]. SCIENTIFIC REPORTS,2016,6(无):1-9.
APA	Fang, Zihui,Xu, An,Wu, Lijun,Hei, Tom K.,&Hong, Mei.(2016).The role of protein kinase C alpha translocation in radiation-induced bystander effect.SCIENTIFIC REPORTS,6(无),1-9.
MLA	Fang, Zihui,et al."The role of protein kinase C alpha translocation in radiation-induced bystander effect".SCIENTIFIC REPORTS 6.无(2016):1-9.