Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification

doi:10.1371/journal.pone.0175050

	Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification
	Fang, Shimeng 2; Tian, Hongzhu 2; Li, Xiancheng 3; Jin, Dong 2; Li, Xiaojie 2; Kong, Jing 2; Yang, Chun 4; Yang, Xuesong 5; Lu, Yao 1; Luo, Yong 6
刊名	PLOS ONE
	2017-04-03
卷号	12
DOI	10.1371/journal.pone.0175050
英文摘要	Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more exosomes in serum than normal cells and these exosomes over-express a certain number of cancer-related biomarkers. However, capture and biomarker analysis of exosomes for clinical application are technically challenging. In this study, we developed a microfluidic chip for immunocapture and quantification of circulating exosomes from small sample volume and applied this device in clinical study. Circulating EpCAM-positive exosomes were measured in 6 cases breast cancer patients and 3 healthy controls to assist diagnosis. A significant increase in the EpCAM-positive exosome level in these patients was detected, compared to healthy controls. Furthermore, we quantified circulating HER2-positive exosomes in 19 cases of breast cancer patients for molecular classification. We demonstrated that the exosomal HER2 expression levels were almost consistent with that in tumor tissues assessed by immunohistochemical staining. The microfluidic chip might provide a new platform to assist breast cancer diagnosis and molecular classification.
语种	英语
WOS记录号	WOS:000399351000071
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152329]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China 2.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 2, Dept Urol, Dalian, Peoples R China 4.Dalian Med Univ, Affiliated Hosp 1, Dept Nucl Med, Dalian, Peoples R China 5.Dalian Med Univ, Dept Biochem & Mol Biol, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dalian, Peoples R China 6.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Fang, Shimeng,Tian, Hongzhu,Li, Xiancheng,et al. Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification[J]. PLOS ONE,2017,12.
APA	Fang, Shimeng.,Tian, Hongzhu.,Li, Xiancheng.,Jin, Dong.,Li, Xiaojie.,...&Liu, Tingjiao.(2017).Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification.PLOS ONE,12.
MLA	Fang, Shimeng,et al."Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification".PLOS ONE 12(2017).