Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis

doi:10.1016/j.chroma.2017.01.029

	Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis
	Liu, Zhongshan 1,2; Liu, Jing 1,2; Liu, Zheyi 1,2; Wang, Hongwei 2; Ou, Junjie 2; Ye, Mingliang 2; Zou, Hanfa 1,2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2017-05-19
卷号	1498 页码:29-36
关键词	Monolithic Column Thiol-ene Click Reaction Proteomics Analysis Two-dimensional Separation Liquid Chromatography
DOI	10.1016/j.chroma.2017.01.029
英文摘要	The vinyl-functionalized hybrid monolithic columns (75 and 150 mu m i.d.) were prepared via solgel chemistry of tetramethoxysilane (TMOS) and vinyltrimethoxysilane (VTMS). The content of accessible vinyl groups was further improved after the monolithic column was post-treated with vinyldimethylethoxysilane (VDMES). The surface properties of monolithic columns were tailored via thiol-ene click reaction by using 1-octadecanethiol, sodium 3-mercapto-l-propanesulfonate and 2,2'-(ethylenedioxy)diethanethiol/vinylphosphonic acid, respectively. The preparing octadecylfunctionalized monolithic columns were adopted for proteomics analysis in cLC-MS/MS. A 37-cm-long x 75-mu m-i.d. monolithic column could identify 3918 unique peptides and 1067 unique proteins in the tryptic digest of proteins from HeLa cells. When a 90-cm-long x 75-mu m-i.d. monolithic column was used, the numbers of unique peptides and proteins were increased by 82% and 32%, respectively. Furthermore, strong cation exchange (SCX) monolithic columns (4 cm in length x 150 mu m i.d.) were also prepared and coupled with the 37-cm-long x 75-mu m-i.d. octadecyl-functionalized monolithic column for two-dimensional" SCX-RPLC-MS/MS analysis, which could identify 17114 unique peptides and 3211 unique proteins. (C) 2017 Elsevier B.V. All rights reserved.
语种	英语
WOS记录号	WOS:000401043000005
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152264]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhongshan,Liu, Jing,Liu, Zheyi,et al. Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1498:29-36.
APA	Liu, Zhongshan.,Liu, Jing.,Liu, Zheyi.,Wang, Hongwei.,Ou, Junjie.,...&Zou, Hanfa.(2017).Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis.JOURNAL OF CHROMATOGRAPHY A,1498,29-36.
MLA	Liu, Zhongshan,et al."Functionalization of hybrid monolithic columns via thiol-ene click reaction for proteomics analysis".JOURNAL OF CHROMATOGRAPHY A 1498(2017):29-36.