Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system

doi:10.1016/j.snb.2018.02.096

	Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system
	Yan, Xiaolu 2; Zhao, Xian-En 2; Sun, Jing 1; Zhu, Shuyun 1,2; Lei, Cuihua 2; Li, Ru 2; Gong, Peiwei 2; Ling, Baoping 2; Wang, Renjun 3; Wang, Hua 2
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-06-15
卷号	263 页码:27-35
关键词	Graphene Quantum Dots Gold Nanoparticles Enzyme Catalysis Glutathione Reductase Inhibitor Screening
DOI	10.1016/j.snb.2018.02.096
英文摘要	Graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) were combined for probing glutathione reductase (GR) activity. The main probing mechanism is based on the following facts: (1) The fluorescence of GQDs can be quenched by AuNPs through fluorescence resonance energy transfer (FRET). (2) Reduced glutathione (GSH) can protect AuNPs from being aggregated and enlarge the inter-particle distance between GQDs and AuNPs, which subsequently produces the fluorescent signal recovery. (3) AuNPs has much weaker affinity towards oxidized glutathione (GSSG) in contrast to GSH. (4) GR can catalyze the reduction of GSSG into GSH in the presence of coenzyme beta-nicotinamide adenine dinucleotide 2'-phophate hydrate (NADPH). Based on these facts, a turn-on fluorescent assay for GR has thus been developed based on the GQDs-AuNPs system. The proposed assay can allow for the evaluation of GR activity in the range of 0.0050-0.13 mU/mL, with a minimum detectable concentration of 0.0050 mU/mL, which was more than one order of magnitude lower than most of the current sensing platforms. Besides, this GQDs-AuNPs based GR assay was successfully applied for screening the inhibitor of GR activity by using 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) as a model. (C) 2018 Elsevier B.V. All rights reserved.
语种	英语
WOS记录号	WOS:000427704400004
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13342]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Inst Med & Mat Appl Technol, Qufu City 273165, Shandong, Peoples R China 3.Qufu Normal Univ, Coll Life Sci, Qufu City 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yan, Xiaolu,Zhao, Xian-En,Sun, Jing,et al. Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,263:27-35.
APA	Yan, Xiaolu.,Zhao, Xian-En.,Sun, Jing.,Zhu, Shuyun.,Lei, Cuihua.,...&Wang, Hua.(2018).Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system.SENSORS AND ACTUATORS B-CHEMICAL,263,27-35.
MLA	Yan, Xiaolu,et al."Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system".SENSORS AND ACTUATORS B-CHEMICAL 263(2018):27-35.