A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts

doi:10.1039/c7an00150a

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院合肥智能机械研究所

	A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts
	Ning, Dianhua 1,2; He, Changtian 1,2; Liu, Zhengjie 1,2; Liu, Cui 1,2; Wu, Qilong 1,2; Zhao, TingTing 1,3; Liu, Renyong 1,2,3
刊名	ANALYST
	2017-05-21
卷号	142 期号:10 页码:1697-1702
DOI	10.1039/c7an00150a
文献子类	Article
英文摘要	Human telomerase RNA (hTR), which is one component of telomerase, was deemed to be a biomarker to monitor tumor cells due to its different expression levels in tumor cells and normal somatic cells. Thus far, plentiful fluorescent probes have been designed to investigate nucleic acids. However, most of them are limited since they are time-consuming, require professional operators and even result in false positive signals in the cellular environment. Herein, we report a dual-colored ratiometric-fluorescent oligonucleotide probe to achieve the reliable detection of human telomerase RNA in cell extracts. The probe is constructed using a dual-labeled fluorescent oligonucleotide hybridized with target-complemented Dabcyl-labeled oligonucleotide. In the presence of the target, the dual-labeled fluorescent oligonucleotide translates into a hairpin structure, which leads to the generation of the fluorescence resonance energy transfer (FRET) phenomenon under UV excitation. Compared to conventional methods, this strategy could effectively avoid false positive signals, and it not only possesses the advantages of simplicity and high specificity but also has the merits of signal stability and distinguishable color variation. Moreover, the quantitative assay of hTR would have a far-reaching impact on the telomerase mechanism and even tumor diagnosis research.
WOS关键词	RESONANCE ENERGY-TRANSFER ; HYBRIDIZATION CHAIN-REACTION ; MESSENGER-RNA ; QUANTUM DOTS ; POLYACRYLAMIDE-GELS ; GRAPHENE OXIDE ; FRET ; DNA ; HTR ; EXPRESSION
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000401526100007
资助机构	National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; National Natural Science Foundation of China(21335006 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21475135 ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131) ; 21375131)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/31863]
专题	合肥物质科学研究院_中科院合肥智能机械研究所
作者单位	1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Ning, Dianhua,He, Changtian,Liu, Zhengjie,et al. A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts[J]. ANALYST,2017,142(10):1697-1702.
APA	Ning, Dianhua.,He, Changtian.,Liu, Zhengjie.,Liu, Cui.,Wu, Qilong.,...&Liu, Renyong.(2017).A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts.ANALYST,142(10),1697-1702.
MLA	Ning, Dianhua,et al."A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts".ANALYST 142.10(2017):1697-1702.