Backbone H-1, C-13 and N-15 resonance assignments of dengue virus NS2B-NS3p in complex with aprotinin

doi:10.1007/s12104-012-9395-9

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	Backbone H-1, C-13 and N-15 resonance assignments of dengue virus NS2B-NS3p in complex with aprotinin
	Bi, Yunchen 1; Zhu, Lei 1; Li, Hua 2; Wu, Bo 1; Liu, Jinsong 2; Wang, Junfeng 1,3
刊名	BIOMOLECULAR NMR ASSIGNMENTS
	2013-10-01
卷号	7 期号:2 页码:137-139
关键词	Dengue Virus Complex Ns2b-ns3p Inhibitor Assignment Nmr
DOI	10.1007/s12104-012-9395-9
文献子类	Article
英文摘要	Dengue virus, belongs to Flaviviridae, is an arthropod transmitted virus that threatens millions of people's lives. As with other flaviviruses, a positive single-stranded 11-kilobases RNA in the dengue virus genome encodes three structural proteins (capsid protein C, membrane protein M, and envelope protein E) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The two component protease NS2B-NS3p is essential for viral replication and is believed to be a potential antiviral drug target. Aprotinin, a native inhibitor, is proved to retard the activity of NS2B-NS3p. The backbone assignments of NS2B-NS3p will be essential for determining the high resolution solution structure of NS2B-NS3p and screening new antiviral drugs. Herein, we report the backbone H-1, N-15, C-13 resonance assignments of the N terminal fragment of NS2B (4.8 kDa) and NS3p (18.5 kDa) in complex with aprotinin (6.5 kDa) by high resolution NMR.
WOS关键词	CHEMICAL-SHIFT ; LARGE PROTEINS ; NMR STRUCTURES ; TROSY ; NS2B ; NS3
WOS研究方向	Biophysics ; Spectroscopy
语种	英语
WOS记录号	WOS:000323740300005
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22197]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Chinese Acad Sci, High Field Magnet Lab, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Ctr Med Phys & Technol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Bi, Yunchen,Zhu, Lei,Li, Hua,et al. Backbone H-1, C-13 and N-15 resonance assignments of dengue virus NS2B-NS3p in complex with aprotinin[J]. BIOMOLECULAR NMR ASSIGNMENTS,2013,7(2):137-139.
APA	Bi, Yunchen,Zhu, Lei,Li, Hua,Wu, Bo,Liu, Jinsong,&Wang, Junfeng.(2013).Backbone H-1, C-13 and N-15 resonance assignments of dengue virus NS2B-NS3p in complex with aprotinin.BIOMOLECULAR NMR ASSIGNMENTS,7(2),137-139.
MLA	Bi, Yunchen,et al."Backbone H-1, C-13 and N-15 resonance assignments of dengue virus NS2B-NS3p in complex with aprotinin".BIOMOLECULAR NMR ASSIGNMENTS 7.2(2013):137-139.