Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides | |
Li, Liping3; Chen, Shuai2; Liu, Huwei1; Qi, Limin2; Bai, Yu1; Ma, Wen1; Zhang, Feng2 | |
刊名 | ACS APPLIED MATERIALS & INTERFACES |
2016-12-28 | |
卷号 | 8期号:51页码:35099-35105 |
关键词 | phosphopeptides endogenous peptides multifunctional affinity probe sequential enrichment mass spectrometry |
英文摘要 | A novel multifunctional composite comprising mesocrystalline SnO, nanorods (NRs) vertically aligned on reduced graphene oxide (rGO) sheets was synthesized and developed for sequential capture of endogenous peptides and phosphopeptides. With the hydrophobicity of rGO and high affinity of SnO2 nanorods, sequential enrichment of endogenous peptides and phosphopeptides could be easily achieved through a modulation of elution buffer. With this multifunctional nanomaterial, 36 peptides were observed from diluted bovine serum albumin (BSA) tryptic digest and 4 phosphopeptides could be selectively captured from beta-casein digest. The detection limit of tryptic digest of beta-casein was low to 4 X 10(-10) M, and the selectivity was up to 1:500 (molar ratio of beta-casein and BSA digest). The effectiveness and robustness of rGO-SnO2 NRs in a complex biological system was also confirmed by using human serum as a real sample. Our work is promising for small peptide enrichment and identification especially in complicated biological sample preparation, which also opens a new perspective in the design of multifunctional affinity probes for proteome or peptidome. |
WOS标题词 | Science & Technology ; Technology |
类目[WOS] | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
关键词[WOS] | LITHIUM-ION BATTERIES ; PHOSPHORYLATION SITES ; CANCER BIOMARKERS ; SELECTIVE CAPTURE ; MASS-SPECTROMETRY ; MS ANALYSIS ; MICROSPHERES ; PROTEIN ; CARBON ; MATRIX |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000391081700017 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/151848] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem, State Key Lab Struct Chem Stable & Unstable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 3.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Liping,Chen, Shuai,Liu, Huwei,et al. Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35099-35105. |
APA | Li, Liping.,Chen, Shuai.,Liu, Huwei.,Qi, Limin.,Bai, Yu.,...&Zhang, Feng.(2016).Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides.ACS APPLIED MATERIALS & INTERFACES,8(51),35099-35105. |
MLA | Li, Liping,et al."Facile Synthesis of Mesocrystalline SnO2 Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides".ACS APPLIED MATERIALS & INTERFACES 8.51(2016):35099-35105. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论